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Arzneimittelforschung 2011; 61(8): 477-480
DOI: 10.1055/s-0031-1296230
Antibiotics · Antimycotics · Antiparasitics · Antiviral Drugs · Chemotherapeutics · Cytostatics
Editio Cantor Verlag Aulendorf (Germany)

Rapid high-performance liquid chromatographic method for determination of adefovir in plasma using UV detection: application to pharmacokinetic studies

Foroutan Seyed Mohsen
1   Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Zarghi Afshin
2   Department of Pharmaceutical Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Shafaati Alireza
2   Department of Pharmaceutical Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Movahed Hooman
3   Noor Research and Educational Institute, Tehran, Iran
,
Khoddam Arash
3   Noor Research and Educational Institute, Tehran, Iran
› Author Affiliations
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Publication History

Publication Date:
27 November 2011 (online)

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Abstract

A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of adefovir (CAS 106941-25-7) in human plasma. The separation was achieved on a monolithic silica column (Chromolith Performance RP-18e, 10064.6 mm) using acetonitrile-ammoniumdihydrogen phosphate buffer (6: 94, v/v), pH 5.2, as the mobile phase at a flow rate of 1.5 ml min–1. The wavelength was set at 260 nm. The assay enables the measurement of adefovir for therapeutic drug monitoring with a minimum quantification limit of 1 ng ml–1. The method involves a simple protein precipitation procedure. Analytical recovery was complete. The calibration curve was linear over the concentration range 1–40 ng ml–1. The coefficients of variation for inter-day and intra-day assay were found to be less than 5%. The method was applied to the determination of adefovir in plasma from 12 subjects dosed with adefovir 2610 mg tablets and pharmacokinetic parameters were evaluated.

Key words

Adefovir - HPLC - Nucleotide analog reverse transcriptase inhibitor - Pharmacokinetic studies - Plasma concentration
 

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