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Exp Clin Endocrinol Diabetes 2011; 119(7): 451-455
DOI: 10.1055/s-0031-1279740
Short Communication

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Insulin Resistance in Non-Obese Women with Polycystic Ovary Syndrome: Relation to Byproducts of Oxidative Stress

D. Macut1 , T. Simic2 , A. Lissounov3 , M. Pljesa-Ercegovac2 , I. Bozic1 , T. Djukic2 , J. Bjekic-Macut4 , M. Matic2 , M. Petakov1 , S. Suvakov2 , S. Damjanovic1 , A. Savic-Radojevic2
  • 1Institute of Endocrinology, Diabetes and Metabolic Diseases, Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
  • 2Institute of Medical and Clinical Biochemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
  • 3Faculty of Medicine, University of Belgrade, Belgrade, Serbia
  • 4Department of Endocrinology, CHC Bezanijska kosa, Belgrade, Serbia
Further Information

Publication History

received 17.12.2010 first decision 20.04.2011

accepted 12.05.2011

Publication Date:
10 June 2011 (online)

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Abstract

To get more insight into molecular mechanisms underlying oxidative stress and its link with insulin resistance, oxidative stress parameters, as well as, antioxidant enzyme activities were studied in young, non-obese women with polycystic ovary syndrome (PCOS). Study was performed in 34 PCOS women and 23 age and body mass index (BMI)-matched healthy controls. Plasma nitrotyrosine and malondialdehyde (MDA), representative byproducts of protein and lipid oxidative damage, were determined by enzyme immunoassay. Antioxidant enzyme activities, superoxide dismutase (SOD) and glutathione peroxidase (GPX) were studied spectrophotometrically. Insulin resistance was calculated using homeostasis assessment model (HOMA-IR). Plasma nitrotyrosine and MDA were increased, but only nitrotyrosine was signifi cantly higher (p < 0.05) in PCOS women compared to controls. Uric acid (surrogate marker of × antine oxidase) was also signifi – cantly elevated in PCOS (p < 0.05). Both plasma SOD and GPX activity showed no statistically significant difference between PCOS and controls. Indices of insulin resistance (insulin and HOMAIR) were significantly higher in PCOS group and positively correlated with level of MDA (r = 0.397 and r = 0.523, respectively; p < 0.05) as well as GPX activity (r = 0.531 and r = 0.358, respectively; p < 0.05). Our results indicate that insulin resistance could be responsible for the existence of subtle form of oxidative stress in young, nonobese PCOS women. Hence, presence of insulin resistance, hyperinsulinemia and oxidative damage are likely to accelerate slow development of cardiovascular disease in PCOS.

Key words

polycystic ovary syndrome - oxidative stress - nitrotyrosine - lipid peroxidation

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Correspondence

A.  Savic-RadojevicMD, PhD 

Assistant Professor of

Biochemistry

Institute of Medical and Clinical

Biochemistry

Faculty of Medicine

University of Belgrade

Pasterova 2

11000 Belgrade

Serbia

Phone: + 381/11/3643 254

Fax: + 381/11/3643 270

Email: ana.savicradojevic@med.bg.ac.rs

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