Differential Expression of Alternative Acyl-CoA Binding Protein (ACBP) Transcripts in an Inducible Human Preadipocyte Cell Line

A. H. Ludewig; M. Klapper; M. Wabitsch; F. Döring; I. Nitz

doi:10.1055/s-0031-1273768

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2011; 43(6): 440-442
DOI: 10.1055/s-0031-1273768

Short Communication

Differential Expression of Alternative Acyl-CoA Binding Protein (ACBP) Transcripts in an Inducible Human Preadipocyte Cell Line

A. H. Ludewig¹ , M. Klapper¹ , M. Wabitsch² , F. Döring¹ , I. Nitz¹

¹Institute of Human Nutrition and Food Science, Research Group Molecular Prevention, University of Kiel, Kiel, Germany
²Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, University of Ulm, Ulm, Germany

Abstract

Understanding the function of fat metabolism during differentiation of human preadipocytes to fully developed fat tissue has been the aim of various studies in the past decades. Due to the lack of suitable human cell culture lines, experimental research predominantly focused on rodent models and nonhuman cell culture systems. Here, we demonstrate that a human preadipocyte cell line SGBS is well suited to examine differential expression of the Acyl-CoA binding protein (ACBP) during adipogenesis. The Acbp gene expresses various alternative high- and low-abundant transcript variants encoding ACBP protein isoforms, which play a central role in fat metabolism. Whereas the low-abundant transcript Acbp-1G is downregulated during SGBS adipogenesis, the high-abundant and well established transcripts Acbp-1A (1) and -1B are moderately (2–4-fold) upregulated. In contrast, the alternative high-abundant transcript Acbp-1C is strongly (29-fold) upregulated at mRNA and protein level indicating that particularly ACBP-1C functions in lipogenic processes during fat cell differentiation in humans.

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Referenzen

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Correspondence

F. Döring

Institute of Human Nutrition

and Food Science

Research Group Molecular

Prevention

University of Kiel

Heinrich-Hecht Platz 10

24098 Kiel

Germany

Telefon: +49/431/880 5699

Fax: +49/431/880 4699

eMail: fdoering@molprev.uni-kiel.de