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DOI: 10.1055/s-0030-1267174
© Georg Thieme Verlag KG Stuttgart · New York
Combined Effects of Short-term Calorie Restriction and Exercise on Insulin Action in Normal Rats
Publication History
received 10.05.2010
accepted 31.08.2010
Publication Date:
11 October 2010 (online)

Abstract
The present study examined the effect of combination of short-term calorie restriction (CR) and moderate exercise on insulin action in normal rats. Rats were divided randomly into 4 groups: ad libitum, sedentary (A-Sed); calorie restriction, sedentary (CR-Sed); ad libitum, exercise (A-Ex); and calorie restriction, exercise (CR-Ex). Rats in the exercise groups were run on a rodent treadmill. Rats in the CR groups were fed every alternate day. Oral glucose tolerance test (OGTT) showed improvements in both CR-Sed and A-Ex groups compared with the A-Sed group; no further improvement in glucose tolerance was observed in the CR-Ex group. In contrast, glucose infusion rates (GIRs) determined by the hyperinsulinemic-euglycemic clamp method indicated that the GIR of the CR and exercise combination was significantly better than that of the sole intervention of CR or exercise. There was no difference in the levels of fasting glucose, insulin, or high-molecular weight forms of adiponectin among the 4 groups. Protein expression of GLUT-4 in the skeletal muscle increased by exercise, but not by CR. Our findings indicate that the combination of exercise and CR may be effective in enhancing insulin sensitivity at the skeletal muscle in normal subjects.
Key words
insulin action - euglycemic clamp method - calorie restriction - exercise - glucose transporter
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Correspondence
Y. OshidaMD, PhD
Department of Sports Medicine
Graduate School of Medicine
Nagoya University
Nagoya 464-8601
Japan
Phone: +81/52/789 3961
Fax: +81/52/789 3957
Email: oshida@htc.nagoya-u.ac.jp