Background and study aims: Bacterial contamination of endoscopy suites is of concern; however studies evaluating
bacterial aerosols are lacking. We aimed to determine the effectiveness of air suctioning
during removal of biopsy forceps in reducing bacterial air contamination.
Patients and methods: This was a prospective single-blinded trial involving 50 patients who were undergoing
elective nontherapeutic colonoscopy. During colonoscopy, endoscopists removed the
biopsy forceps first without and then with suctioning following contact with the sigmoid
mucosa. A total of 50 L of air was collected continuously for 30 seconds at 30-cm
distance from the biopsy channel valve of the colonoscope, with time starting at forceps
removal. Airborne bacteria were collected by an impactor air sampler (MAS-100). Standard
Petri dishes with CNA blood agar were used to culture Gram-positive bacteria. Main
outcome measure was the bacterial load in endoscopy room air.
Results: At the beginning and end of the daily colonoscopy program, the median (and interquartile
[IQR] range) bioaerosol burden was 4 colony forming units (CFU)/m3 (IQR 3 – 6) and 16 CFU/m3 (IQR 13 – 18), respectively. Air suctioning during removal of the biopsy forceps
reduced the bioaerosol burden from a median of 14 CFU/m3 (IQR 11 – 29) to a median of 7 CFU/m3 (IQR 4 – 16) (P = 0.0001). Predominantly enterococci were identified on the agar plates.
Conclusion: The bacterial aerosol burden during handling of biopsy forceps can be reduced by
applying air suction while removing the forceps. This simple method may reduce transmission
of infectious agents during gastrointestinal endoscopies.
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1 The authors contributed equally.
S. R. VavrickaMD
University Hospital Zurich
Division of Gastroenterology and Hepatology
Raemistrasse 100
CH-8091 Zurich
Switzerland
Fax: +41-44-2554503
Email: stephan.vavricka@usz.ch