Abstract
Two new O-prenylated acridone alkaloids, balsacridone A (1) and B (2), together with eighteen known compounds were isolated from the methanol extract
from the stems of Balsamocitrus paniculata, a Cameroonian medicinal plant. The structures of all compounds were determined by
comprehensive analyses of their 1D and 2D NMR, mass spectral (EI and ESI) data, and
chemical reactions. N-methyl-6-methoxybenzoxazolinone (16) was isolated for the first time from a natural source while compounds 13, 14, and 15 for the first time from this genus. Pure compounds were tested for their activity
against bacteria, fungi, and plant pathogen oomycetes, using the paper disk agar diffusion
assay. The agar diffusion test delivered low to missing antimicrobial activities,
corresponding to MICs > 1 mg/mL. However, compounds 1–15 exhibited a strong suppressive effect on phagocytosis response upon activation with
serum opsonized zymosan in the range of IC50 = 0.5–7.2 µM, and the acridone alkaloids (1–5), N-trans-p-coumaroyltyramine (13), and N-trans-p-coumaroyloctopamine (14) displayed weak cytotoxic activity against the human Caucasian prostate adenocarcinoma
cell line PC-3, with IC50 values ranging from 69.8 to 99.0 µM.
Key words
Balsamocitrus paniculata
- Rutaceae - acridone - antimicrobial - oxidative burst inhibition - cytotoxic activities
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Jean Duplex Wansi
Faculty of Science, Department of Chemistry
University of Douala
P. O. Box 24157
Douala
Cameroon
Phone: +23 7 74 72 54 25
Fax: +23 7 33 40 75 69
Email: jdwansi@yahoo.fr