Two New Lignans from the Roots of Pulsatilla koreana

Abstract

Two new lignans, (2R,3R)-2β-(4′′-hydroxy-3′′-methoxybenzyl)-3α-(4′-hydroxy-3′-methoxybenzyl)-γ-butyrolactone 2-O-(β-D-glucopyranoside) (1) and (1S,2R,3S)-dimethyl-1,2,3,4-tetrahydro-3,6,7-trihydroxy-1-(3′,4′-dihydroxyphenyl)naphthalene-2,3-dicarboxylate (2) together with nine known compounds (3–11) were isolated from the ethyl acetate fraction of the roots of Pulsatilla koreana. Their chemical structures were established based on physicochemical and spectroscopic data analyses. All isolates were investigated for their inhibition effects against the classical pathway of the complement system. Among them, compound 6 showed significant inhibitory activity with an IC₅₀ value of 75.9 µM, compounds 8 and 9 had moderate effects with IC₅₀ values of 182.2 and 166.5 µM, respectively.

Key words

Pulsatilla koreana - Ranunculaceae - lignan - anticomplement activity

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