Chemical Constituents of Alnus firma and Their Inhibitory Activity on Lipopolysaccharide-Induced Nitric Oxide Production in BV2 Microglia

Abstract

A methanolic extract of Alnus firma barks (Betulaceae) significantly attenuated nitric oxide production in lipopolysaccharide-stimulated BV2 microglia. Two new compounds, characterized as 4-hydroxy-2,6-dimethoxyphenyl 6′-O-syringoyl-β-D-glucopyranoside (1) and 4-hydroxy-2,6-dimethoxyphenyl 6′-O-vanilloyl-β-D-glucopyranoside (2), were isolated from the barks of A. firma using bioactivity-guided fractionation, together with two known phenolic glycosides (3, 4) and 11 known diarylheptanoids (5–15). Among these compounds, 1–4 and 6–11 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 10 µM to 100 µM.

Key words

Alnus firma (Betulaceae) - phenolic glycosides - diarylheptanoids - nitric oxide - BV2 microglia - lipopolysaccharide

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