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Planta Med 2009; 75(2): 148-151
DOI: 10.1055/s-0028-1088347
Pharmacology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Modulation of Macrophage Functions by Compounds Isolated from Zingiber officinale

Eun Mi Koh1 , Hye Jin Kim2 , Sohee Kim1 , Woo Hyuck Choi1 , Yeon Hee Choi2 , Shi Yong Ryu2 , Young Sup Kim2 , Woo Suk Koh1 , Shin-Young Park1
  • 1Korea Institute of Toxicology, Daejeon, Korea
  • 2Korea Research Institute of Chemical Technology, Daejeon, Korea
Further Information

Publication History

Received: March 31, 2008 Revised: September 1, 2008

Accepted: October 1, 2008

Publication Date:
24 November 2008 (online)

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Abstract

Bioactivity-guided fractionation of Zingiber officinale (Zingiberaceae) led us to isolate 14 compounds, [4]-gingerol (1), [6]-gingerol (2), [8]-gingerol (3), [10]-gingerol (4), [6]-paradol (5), [4]-shogaol (6), [6]-shogaol (7), 1-dehydro-[10]-gingerdione (8), [10]-gingerdione (9), hexahydrocurcumin (10), tetrahydrocurcumin (11), gingerenone A (12), 1,7-bis-(4’ hydroxyl-3’ methoxyphenyl)-5-methoxyhepthan-3-one (13), and methoxy-[10]-gingerol (14). Using the RAW 264.7 cell line, the inhibitory effects on nitric oxide production induced by lipopolysaccharide and the stimulatory effects on phagocytosis of these compounds were evaluated. Compounds 7, 8, and 9 significantly decreased lipopolysaccharide-induced nitric oxide production, and compounds 7 and 8 significantly reduced inducible nitric oxide synthase expression. Among them, compound 8 also showed significant stimulatory effects on phagocytosis.

Abbreviations

Con A:concanavalin A

DMEM:Dulbecco’s modified Eagle’s medium

FBS:fetal bovine serum

iNOS:inducible nitric oxide synthase

LPS:lipopolysaccharide

NO:nitric oxide

TTBS:Tween tris buffered saline

Key words

Zingiber officinale - Zingiberaceae - macrophage - nitric oxide - phagocytic activity

References

  • 1 Underhill D M, Ozinsky A, Hajjar A M, Stevens A, Wilson C B, Bassetti M. et al . The Toll-like receptor 2 is recruited to macrophage phagosomes and discriminates between pathogens.  Nature. 1999;  401 811-5
    CrossrefPubMedSearch in Google Scholar
  • 2 Unanue E R. The mononuclear-phagocytic system. In: Tizard IR, editors Immunology. Saunders; Saunders College Publishing 1994: 61-74
    Search in Google Scholar
  • 3 Heiss E, Herhaus C, Klimo K, Bartsch H, Gerhauser C. Nuclear factor kappa B is a molecular target for sulforaphane-mediated anti-inflammatory mechanisms.  J Biol Chem. 2001;  276 32 008-15
    PubMedSearch in Google Scholar
  • 4 Dawn B, Bolli R. Role of nitric oxide in myocardial preconditioning.  Ann NY Acad Sci. 2002;  962 18-41
    CrossrefPubMedSearch in Google Scholar
  • 5 Bogdan C. Nitric oxide and the immune response.  Nat Immunol. 2001;  2 907-16
    CrossrefPubMedSearch in Google Scholar
  • 6 Appleton I, Tomlinson A, Willoughby D A. Induction of cyclo-oxygenase and nitric oxide synthase in inflammation.  Adv Pharmacol. 1996;  35 27-78
    PubMedSearch in Google Scholar
  • 7 Alderton W K, Cooper C E, Knowles R G. Nitric oxide synthases: structure, function and inhibition.  Biochem J. 2001;  357 593-615
    CrossrefPubMedSearch in Google Scholar
  • 8 Aktan F, Henness S, Tran V H, Duke C C, Roufogalis B D, Ammit A J. Gingerol metabolite and a synthetic analogue Capsarol inhibit macrophage NF-kappaB-mediated iNOS gene expression and enzyme activity.  Planta Med. 2006;  72 727-34
    Thieme ConnectPubMedSearch in Google Scholar
  • 9 Ojewole J A. Analgesic, antiinflammatory and hypoglycaemic effects of ethanol extract of Zingiber officinale (Roscoe) rhizomes (Zingiberaceae) in mice and rats.  Phytother Res. 2006;  20 764-72
    CrossrefPubMedSearch in Google Scholar
  • 10 Masada Y, Inoue T, Hashimoto K, Fujioka M, Shiraki K. Studies on the pungent principles of ginger (Zingiber officinale Roscoe) by GC-MS.  Yakugaku Zasshi. 1973;  93 318-21
    PubMedSearch in Google Scholar
  • 11 Surh Y J. Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review.  Food Chem Toxicol. 2002;  40 1091-7
    CrossrefPubMedSearch in Google Scholar
  • 12 Ippoushi K, Ito H, Horie H, Azuma K. Mechanism of inhibition of peroxynitrite-induced oxidation and nitration by [6]-gingerol.  Planta Med. 2005;  71 563-6
    Thieme ConnectPubMedSearch in Google Scholar
  • 13 Shin S G, Kim J Y, Chung H Y, Jeong J C. Zingerone as an antioxidant against peroxynitrite.  J Agric Food Chem. 2005;  53 7617-22
    CrossrefPubMedSearch in Google Scholar
  • 14 Thomson M, Al-Qattan K K, Al-Sawan S M, Alnaqeeb M A, Khan I, Ali M. The use of ginger (Zingiber officinale Rosc.) as a potential anti-inflammatory and antithrombotic agent.  Prostaglandins Leukot Essent Fatty Acids. 2002;  67 475-8
    CrossrefPubMedSearch in Google Scholar
  • 15 Shukla Y, Singh M. Cancer preventive properties of ginger: a brief review.  Food Chem Toxicol. 2007;  45 683-90
    PubMedSearch in Google Scholar
  • 16 Aktan F. iNOS-mediated nitric oxide production and its regulation.  Life Sci. 2004;  75 639-53
    CrossrefPubMedSearch in Google Scholar
  • 17 Krysko D V, Denecker G, Festjens N, Gabriels S, Parthoens E, D’Herde K. et al . Macrophages use different internalization mechanisms to clear apoptotic and necrotic cells.  Cell Death Differ. 2006;  13 2011-22
    CrossrefPubMedSearch in Google Scholar

Shin-Young Park, PhD.

Korea Institute of Toxicology

100 Jangdong, Yuseong

Daejeon 305–343

Korea

Phone: +82-42-610-8224

Fax: +82-42-610-8172

Email: sypark@kitox.re.kr

    www.thieme-connect.de/ejournals/toc/plantamedica