Flavan-3-ols from Ulmus davidiana var. japonica with Inhibitory Activity on Protein Glycation

Abstract

Four flavan-3-ols, (+)-catechin (1), (+)-catechin 7-O-β-D-apiofuranoside (2), (+)-catechin 7-O-β-D-xylopyranoside (3), (+)-catechin 7-O-β-D-glucopyranoside (4), and proanthocyanidin A-1 (5) as well as three other constituents, isolated from an EtOAc-soluble extract of the stem barks of Ulmus davidiana var. japonica, were evaluated for inhibitory activity against the formation of AGEs. Compounds 1 – 5 exhibited a significant inhibitory activity on the formation of AGEs in an AGEs-BSA assay by specific fluorescence and this was confirmed by an indirect AGEs-ELISA. Moreover, compounds 1 and 5 markedly reduced AGEs-BSA cross-linking to collagen in a dose-dependent manner.

Abbreviations

AGEs:advanced glycation end products

BSA:bovine serum albumin

CC:column chromatography

CD:circular dichroism

Key words

Ulmus davidiana var. japonica - Ulmaceae - flavan-3-ols - AGEs - AGEs-BSA cross-linking

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