Antibacterial Efficacy of Hiora: An Ayurvedic Mouthwash in Children

Abstract

Background Mouthwashes, as a form of antimicrobial delivery system, rank among the safest and most effective vehicles, particularly in the case of young children. This is attributed to their ability to distribute therapeutic components across all accessible oral surfaces, including interproximal areas.

Objective To evaluate the antibacterial efficacy of recently introduced Ayurvedic (Hiora) and triclosan-based mouthwashes among children.

Materials and Methods A total of 45 healthy children aged 10–15 years were randomly assigned to three groups: Herbal mouthwash (Hiora), triclosan-based mouthwash (Kidodent), and normal saline as the control group. Saliva samples were collected pre-rinse, 2 minutes, 30 minutes, and 60 minutes post-rinsing with the study mouthwashes. These samples were then inoculated onto Petri dishes containing blood agar culture media, followed by incubation under both aerobic and anaerobic conditions at 37°C for 48 hours. The resulting bacterial colonies of Streptococcus spp. and Lactobacillus spp. were counted (CFU/ml x 105). Statistical analysis, including ANOVA, Newman Keul’s Post-hoc test, and a two-tailed ‘t’ test, was conducted to determine the significance of the results.

Results The Ayurvedic Hiora mouthwash demonstrated the most substantial reduction in salivary bacterial colony counts of Streptococcus spp. and Lactobacillus spp. with statistically significant results (p<0.01).

Conclusion The Ayurvedic Hiora mouthwash exhibited the highest antibacterial effectiveness, followed by the triclosan-based mouthwash in decreasing order, with saline showing the least efficacy.

Keywords

antibacterial agent - prevention mouthrinse - ayurvedic - traditional - pediatrics

Publication History

Received: 28 February 2024

Accepted: 26 June 2024

Article published online:
02 August 2024

• References

• 1 Deshmukh MA, Dodamani AS, Karibasappa G. et al. Comparative Evaluation of the Efficacy of Probiotic, Herbal and Chlorhexidine Mouthwash on Gingival Health: A Randomized Clinical Trial. J Clin Diagn Res 2017; 11: 13-16
• 2 Sriram K, Jeevanandan G, Kumar MPS. Association between Age, Gender and Severity of Gingivitis among Children between 6-10 Years-A Retrospective Study. Int J Dent Oral Sci 2021; 1: 4105-4108
• 3 Aspalli S, Shetty VS, Devarathnamma MV. et al. Evaluation of the antiplaque and antigingivitis effect of herbal mouthwash in the treatment of plaque-induced gingivitis: A randomized, clinical trial. J Ind Soc Periodontol 2014; 18: 48-52
• 4 Shah SV, Badakar CM, Hugar SM. et al. Antimicrobial Efficacy of Chlorhexidine and Herbal Mouth Rinse on Salivary Streptococcus mutans in Children with Mixed Dentition: A Randomized Crossover Study. Int J Clin Pediatr Dent 2022; 15: 99-103
• 5 Mathew M, Joyshree C, Ratan VJ. et al. Anti-plaque efficacy of Hi-Ora mouth rinse and 0.12% chlorhexidine gluconate in patients with chronic gingivitis: A case-control study. J Oral Maxillofac Pathol 2022; 26: 38-43
• 6 Al-Bayaty FH, Koubaisi AH, Ali NAW. et al. Effect of mouthwash extracted from Salvadora persica (Miswak) on dental plaque formation: A clinical trial. J Med Plant Res 2010; 4: 1446-54
• 7 Kraivaphan P, Amornchat C, Laothumthut T. et al. Investigation of Triclosan retention and dental plaque viability with a Triclosan/PVM/MA copolymer mouth rinse in a Thai population. Southeast Asian J Trop Med Public Health 2009; 40: 840-7
• 8 Kyaw Myint ZC, Zaitsu T, Oshiro A. et al. Risk indicators of dental caries and gingivitis among 10-11-year-old students in Yangon, Myanmar. Int Dent J 2020; 70: 167-175
• 9 da Silva Pde L, Barbosa Tde S, Amato JN. et al. Gingivitis, Psychological Factors and Quality of Life in Children. Oral Health Prev Dent 2015; 13: 227-35
• 10 Doddawad VG, Jose M, Shivananda S. et al. Effectiveness of Menthol and Chlorhexidine Mouth Rinses on Reduction of Plaque, Gingivitis, and Streptococcus mutans Count in Saliva. International Journal of Pharmaceutical Quality Assurance 2023; 14: 105-109
• 11 Gupta Rahul, Om Prakash Yadav, Mohsin Khan S.. et al. Comparative Evaluation of Efficacy of Hiora, Terminalia Chebula and Chlorhexidine as Mouth Wash on Dental Plaque. J Dent Health, Oral Disorders & Therapy 2017; 8: 547-551
• 12 Mahlawat H, Baptist J, Bhat KG. et al. Antimicrobial efficacy of commonly prescribed mouthwashes. Transylvanian Review 2018; 26: 6299-6304
• 13 Subramaniam P, Nandan N. Effect of xylitol, sodium fluoride and triclosan containing mouth rinse on Streptococcus mutans . Contemp Clin Dent 2011; 2: 287-290
• 14 Vedula C, Potharaju SP, Bathula H. et al. Comparison of Effects of Mouthwash Containing Chlorhexidine and Chlorine Dioxide on Salivary Bacteria-A Randomized Control Study. J Pharma Res Int 2021; 33: 356-362
• 15 Yousefimanesh H, Amin M, Robati M. et al. Comparison of the Antibacterial Properties of Three Mouthwashes Containing Chlorhexidine Against Oral Microbial Plaques: An in vitro Study. Jund J Microbiol 2015; 8: 1-7
• 16 Heliawati L, Lestari S, Hasanah U. et al. Phytochemical Profile of Antibacterial Agents from Red Betel Leaf (Piper crocatum Ruiz and Pav) against Bacteria in Dental Caries. Molecules. 2022; 27: 2861
• 17 Kulkarni VV, Damle SG. Comparative evaluation of the efficacy of sodium fluoride, chlorhexidine, and triclosan mouth rinses in reducing the mutans streptococci count in saliva: An in vivo study. J Indian Soc Pedo Prev Dent 2013; 21: 98-104
• 18 Pires JR, Junior CR, Pizzolitto AC. In vitro antimicrobial efficiency of a mouthwash containing triclosan/gantrez and sodium bicarbonate. Braz Oral Res 2017; 21: 342-347
• 19 Bhat N, Mitra R, Reddy JJ. et al. Evaluation of efficacy of Chlorhexidine and a Herbal mouthwash on dental plaque: an in vitro comparative study. Int J Pharm Bio Sci 2013; 4: 625-632