Withanolide Profile and Acetylcholinesterase Inhibitory Activity of Two Argentinean Jaborosa Species^{[ # ]}

Abstract

Acetylcholinesterase (AChE) inhibitors are still an important option for managing symptoms of mild to moderate Alzheimerʼs disease. In this study, we aimed to evaluate the potential in vitro AChE inhibitory activity of two Argentinian endemic Solanaceae species, Jaborosa bergii and J. runcinata. UHPLC-DAD-HRMS metabolite profiling revealed the presence of withanolides in the active CH₂Cl₂ subextracts. Their fractionation led to the isolation and identification of two known spiranoid withanolides from J. runcinata and three new withanolides with a skeleton similar to that of trechonolide-type withanolides from J. bergii. The known compounds showed moderate AChE inhibitory activity, while the new ones were inactive.

Keywords

Withanolides - Jaborosa bergii - Jaborosa runcinata - Solanaceae - Alzheimerʼs disease - cholinesterase inhibition

^# This work is dedicated to Professors Rudolf Bauer, Chlodwig Franz, Brigitte Kopp, and Hermann Stuppner for their invaluable contributions and commitment to Austrian Pharmacognosy.

Publikationsverlauf

Eingereicht: 29. September 2023

Angenommen nach Revision: 23. Januar 2024

Artikel online veröffentlicht:
06. Juni 2024

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