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DOI: 10.1055/a-2189-7272
Comparative Morphology of Island and Inland Agastache rugosa and Their Gastroprotective Effects in EtOH/HCl-Induced Gastric Mucosal Gastritis
This research was supported by grants for the Development of Sustainable Application for Standard Herbal Resources from the Korea Institute of Oriental Medicine, Republic Korea (KSN1822320) and the Jeju Institute of Korean Medicine through the ‘Industrialization Utilization-Based Research Project of Jeju Dongjeong-gyul and Gwakhyang’ (ERT2011180).
Abstract
Agastache rugosa Kuntze (Lamiaceae; Labiatae), a medicinal and functional herb used to treat gastrointestinal diseases, grows well both on islands and inland areas in South Korea. Thus, we aimed to reveal the morphological and micromorphological differences between A. rugosa grown on island and inland areas and their pharmacological effects on gastritis in an animal model by combining morphological and mass spectrophotometric analyses. Morphological analysis showed that island A. rugosa had slightly smaller plants and leaves than inland plants; however, the density of all types of trichomes on the leaves, petioles, and stems of island A. rugosa was significantly higher than that of inland plants. The essential oil component analysis revealed that pulegone levels were substantially higher in island A. rugosa than in inland A. rugosa. Despite the differences between island and inland A. rugosa, treatment with both island and inland A. rugosa reduced gastric damages by more than 40% compared to the gastritis induction group. In addition, expression of inflammatory protein was reduced by about 30% by treatment of island and inland A. rugosa. The present study demonstrates quantitative differences in morphology and volatile components between island and inland plants; significant differences were not observed between the gastritis-inhibitory effects of island and inland A. rugosa, and the efficacy of island A. rugosa was found to be similar to that of A. rugosa grown in inland areas.
Key words
Agastache rugosa - Lamiaceae - Agastachis Herba - scanning electron microscopy - pulegone - gastrointestinal diseases - gastric mucosal inflammationSupporting Information
- Supporting Information
The whole membrane of the COX-2 and β-actin proteins expression (Fig. 1S) is in the Supporting Information. The results of the original GCMS chromatograms are presented in the Supporting Information (see Fig. 2S).
Publication History
Received: 20 February 2023
Accepted after revision: 28 September 2023
Article published online:
30 October 2023
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References
- 1 Korea Food and Drug Administration. The Korean Herbal Pharmacopoeia. 4th ed. Seoul, Korea: The KFDA Notification; 2020
- 2 Park CW. Agastache . In: Flora of Korea. Asteridae: Loganiaceae to Oleaceae. Vol. 6a. Seoul, Korea: Flora of Korea Editorial Committee; 2018: 93
- 3 Ko SJ, Han G, Kim SK, Seo JG, Chung WS, Ryu B, Kim J, Yeo I, Lee BJ, Lee JM, Park JW. Effect of Korean herbal medicine combined with a probiotic mixture on diarrhea-dominant irritable bowel syndrome: A double-blind, randomized, placebo-controlled trial. Evid Based Complement Alternat Med 2013; 2013: 824605
- 4 Nam HH, Kim JS, Lee J, Seo YH, Kim HS, Ryu SM, Choi G, Moon BC, Lee AY. Pharmacological effects of Agastache rugosa against gastritis using a network pharmacology approach. Biomolecules 2020; 10: 1298
- 5 Seo YH, Kang SY, Shin JS, Ryu SM, Lee AY, Choi G, Moon BC, Jang DS, Shim SH, Lee D, Lee KT, Lee J. Chemical constituents from the aerial parts of Agastache rugosa and their inhibitory activities on prostaglandin E(2) production in lipopolysaccharide-treated RAW 264.7 macrophages. J Nat Prod 2019; 82: 3379-3385
- 6 Seo H, Kim C, Kim MB, Hwang JK. Anti-photoaging effect of Korean mint (Agastache rugosa Kuntze) extract on UVB-irradiated human dermal fibroblasts. Prev Nutr Food Sci 2019; 24: 442-448
- 7 Park CH, Yeo HJ, Baskar TB, Park YE, Park JS, Lee SY, Park SU. In vitro antioxidant and antimicrobial properties of flower, leaf, and stem extracts of Korean mint. Antioxidants (Basel) 2019; 8: 75
- 8 Oh Y, Lim HW, Huang YH, Kwon HS, Jin CD, Kim K, Lim CJ. Attenuating properties of Agastache rugosa leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismutase in a human keratinocyte cell line. J Photochem Photobiol B 2016; 163: 170-176
- 9 Chang Y, Tian Y, Zhou D, Yang L, Liu TM, Liu ZG, Wang SW. Gentiopicroside ameliorates ethanol-induced gastritis via regulating MMP-10 and pERK1/2 signaling. Int Immunopharmacol 2021; 90: 107213
- 10 Kim YS, Park H, Song J, Lee D, Kim H. Antiulcer effects of HT074 on HCl/EtOH induced gastric injury. Korean J Herbol 2018; 33: 9-18
- 11 İpek BE, Yüksel M, Cumbul A, Ercan F, Cabadak H, Aydın B, Alican İ. The effect of metformin on ethanol- and indomethacin-induced gastric ulcers in rats. Turk J Gastroenterol 2022; 33: 767-776
- 12 Rocco A, Compare D, Angrisani D, Sanduzzi Zamparelli M, Nardone G. Alcoholic disease: Liver and beyond. World J Gastroenterol 2014; 20: 14652-14659
- 13 Teyssen S, Singer MV. Alcohol-related diseases of the oesophagus and stomach. Best Pract Res Clin Gastroenterol 2003; 17: 557-573
- 14 Liu M, Hu Z, Wang C, Zhang Y. The TLR/My88 signaling cascade in inflammation and gastric cancer: The immune regulatory network of Helicobacter pylori . J Mol Med (Berl) 2023; 101: 767-781
- 15 Ito N, Tsujimoto H, Ueno H, Xie Q, Shinomiya N. Helicobacter pylori-mediated immunity and signaling transduction in gastric cancer. J Clin Med 2020; 9: 3699
- 16 Cuzzolin L, Locci C, Chicconi E, Antonucci R. Clinical use of gastric antisecretory drugs in pediatric patients with gastroesophageal reflux disease: A narrative review. Transl Pediatr 2023; 12: 260-270
- 17 Wang CH, Chen II, Chen CH, Tseng YT. Pharmacoepidemiological research on N-Nitrosodimethylamine-contaminated ranitidine use and long-term cancer risk: A population-based longitudinal cohort study. Int J Environ Res Public Health 2022; 19: 12469
- 18 Marrelli M, Conforti F, Toniolo C, Nicoletti M, Statti G, Menichini F. Hypericum perforatum: Influences of the habitat on chemical composition, photo-induced cytotoxicity, and antiradical activity. Pharm Biol 2014; 52: 909-918
- 19 Karahan F, Avsar C, Ozyigit II, Berber I. Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats. Biotechnol Biotechnol Equip 2016; 30: 797-804
- 20 Elsharkawy ER, Alghanem SM, Elmorsy E. Effect of habitat variations on the chemical composition, antioxidant, and antimicrobial activities of Achillea fragrantissima (Forssk) Sch. Bip. Biotechnol Rep (Amst) 2021; 29: e00581
- 21 Kim HJ, Jeong HS, Kang SH. Ethnobotany of Jeju Island, Korea. Korean J Plant Res 2015; 28: 217-234
- 22 Gershenzon J, Maffei M, Croteau R. Biochemical and histochemical localization of monoterpene biosynthesis in the glandular trichomes of spearmint (Mentha spicata). Plant Physiol 1989; 89: 1351-1357
- 23 McCaskill D, Gershenzon J, Croteau R. Morphology and monoterpene biosynthetic capabilities of secretory cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L.). Planta 1992; 187: 445-454
- 24 Bertea CM, Schalk M, Karp F, Maffei M, Croteau R. Demonstration that menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: Cloning, functional expression, and characterization of the responsible gene. Arch Biochem Biophys 2001; 390: 279-286
- 25 Božović M, Ragno R. Calamintha nepeta (L.) savi and its main essential oil constituent pulegone: Biological activities and chemistry. Molecules 2017; 22: 290
- 26 Zhou Y, Tang N, Huang L, Zhao Y, Tang X, Wang K. Effects of salt stress on plant growth, antioxidant capacity, glandular trichome density, and volatile exudates of Schizonepeta tenuifolia Briq. Int J Mol Sci 2018; 19: 252
- 27 Su YH, Lin JY. Menthone supplementation ameliorates systemic allergic inflammation in asthmatic mice via regulating Th2-skewed immune balance. Phytomed Plus 2022; 2: 100322
- 28 Yang Q, Liu Q, Lv H, Wang F, Liu R, Zeng N. Effect of pulegone on the NLPR3 inflammasome during inflammatory activation of THP-1 cells. Exp Ther Med 2020; 19: 1304-1312
- 29 Yu L, Li R, Liu W, Zhou Y, Li Y, Qin Y, Chen Y, Xu Y. Protective effects of wheat peptides against ethanol-induced gastric mucosal lesions in rats: Vasodilation and anti-inflammation. Nutrients 2020; 12: 2355
- 30 Bhandari P, Bateman AC, Mehta RL, Patel P. Mucosal expression of cyclooxygenase isoforms 1 and 2 is increased with worsening damage to the gastric mucosa. Histopathology 2005; 46: 280-286
- 31 Hwang HS, Han KJ, Ryu YH, Yang EJ, Kim YS, Jeong SY, Lee YS, Lee MS, Koo ST, Choi SM. Protective effects of electroacupuncture on acetylsalicylic acid-induced acute gastritis in rats. World J Gastroenterol 2009; 15: 973-977
- 32 Fan DD, Lin S, Song YP, Wang ZY, Liu B, Gao SN, Fan YH, Zhu S, Li S, Jiang L. Astragaloside IV protects rat gastric mucosa against aspirin-induced damage. Int Immunopharmacol 2016; 41: 47-55
- 33 Oak MK, Yang S, Choi G, Song JH. Comparative floral and pollen micromorphology of Leonurus japonicus and L. macranthus (Lamiaceae). Diversity (Basel) 2021; 13: 533
- 34 Moon YG, Hong JS, Song MH. DPPH radical scavenging activity and composition of essential oil from the herbs of Jeju Agastache rugosa . J Life Sci 2012; 22: 156-160