Steroidal Saponins from the Rhizomes of Smilax china and Their Inhibitory Effects on Lipopolysaccharide-Induced Proinflammatory Cytokines Expression

Abstract

Four new furostanol saponins (1 – 4) and a new pregane-type saponin (5) along with six known steroidal saponins (6 – 11) were isolated from the rhizomes of Smilax china. The structures of 1 – 5 were elucidated by extensive analysis of NMR and HR-ESI-MS data in addition to enzymatic hydrolysis and other chemical methods. Compounds 1, 4, and 11 showed inhibitory activity against the expression of proinflammatory mediators, inducible nitric oxide synthase, interleukin-1β, interleukin-6, and tumor necrosis factor-α in lipopolysaccharide-induced RAW264.7 cells. Compound 1, at a concentration of 20 µM, decreased the production of inducible nitric oxide synthase, interleukin-1β, interleukin-6, and tumor necrosis factor-α by 36, 62, 72, and 67%, respectively, which is comparable to that of the positive control dexamethasone.

Key words

Smilax china - Smilacaceae - steroidal saponins - anti-inflammatory activity

Publikationsverlauf

Eingereicht: 29. Januar 2022

Angenommen nach Revision: 17. Juni 2022

Artikel online veröffentlicht:
28. September 2022

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