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Planta Med 2022; 88(07): 527-537
DOI: 10.1055/a-1458-5646
Biological and Pharmacological Activity
Original Papers

Low Piperine Fractional Piper nigrum Extract Enhanced the Antitumor Immunity via Regulating the Th1/Th2/Treg Cell Subsets on NMU-Induced Tumorigenesis Rats

Jirakrit Saetang
1   Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
2   Department of Surgery, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
4   EZ-Mol-Design Laboratory, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
,
Aman Tedasen
1   Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
3   The Excellent Research Laboratory of Cancer Molecular Biology, Prince of Songkla University, Songkhla, Thailand
,
Surasak Sangkhathat
2   Department of Surgery, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
,
Natnaree Sangkaew
1   Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
,
Sirinapa Dokduang
1   Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
3   The Excellent Research Laboratory of Cancer Molecular Biology, Prince of Songkla University, Songkhla, Thailand
,
Napat Prompat
1   Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
,
Siriporn Taraporn
1   Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
3   The Excellent Research Laboratory of Cancer Molecular Biology, Prince of Songkla University, Songkhla, Thailand
,
Potchanapond Graidist
1   Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand
3   The Excellent Research Laboratory of Cancer Molecular Biology, Prince of Songkla University, Songkhla, Thailand
› Author AffiliationsSupported by: Agricultural Research Development Agency (Public Organization) CRP6205031530
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Abstract

Cancer is one of the major causes of death worldwide. In addition to standard regimens, tumor suppression ability has been demonstrated in many types of natural products, including Piper nigrum, or black pepper. In previous reports, we demonstrated the antitumor effect of low piperine fractional Piper nigrum extract in vitro and in vivo. However, the effects of low piperine fractional P. nigrum extract in the aspect of antitumor immunity has not yet been investigated. In this study, tumor-bearing rats were fed with 100 mg/kg BW or 200 mg/kg BW of low piperine fractional P. nigrum extract 3 times per week for 4 weeks. Tumor burden and hematological data were then evaluated. Immunological data was investigated using a cytokine array and flow cytometry. The results showed that both doses of low piperine fractional P. nigrum extract significantly suppressed tumor progression in N-nitrosomethylurea-induced mammary tumor rats. There were no significant changes observed in the total white blood cells, red blood cells, and hemoglobin. Low piperine fractional P. nigrum extract suppressed some cytokine and chemokine levels including CXCL7, sICAM-1, and L-selectin 0.2- to 0.6-fold. Interestingly, 200 mg/kg BW of low piperine fractional P. nigrum extract significantly promoted type 1 T helper cell, and suppressed neutrophil, basophil, type 2 T helper cell, and regulatory T cell compared to the control group. In summary, these results indicate that low piperine fractional P. nigrum extract had a high efficacy in supporting antitumor activity at immunological levels via regulating Th1/Th2/Treg cells.

Key words

Piperaceae - Piper nigrum - antitumor immunity - Th1 - Th2 - Treg - PFPE

Supporting Information

    Contents of compound and chromatogram pattern of PFPE analyzed by GM-MS are available as Supporting Information.

  • Supporting Information


Publication History

Received: 16 December 2020

Accepted after revision: 20 March 2021

Article published online:
26 April 2021

© 2021. Thieme. All rights reserved.

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