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Planta Med 2021; 87(09): 709-715
DOI: 10.1055/a-1324-3570
Biological and Pharmacological Activity
Original Papers

Artocarpus lacucha Extract and Oxyresveratrol Inhibit Glucose Transporters in Human Intestinal Caco-2 Cells

Matusorn Wongon
Department of Pharmacy Practice and Center of Excellence for Innovation in Chemistry, Pharmacological Research Unit, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand
,
Nanteetip Limpeanchob
Department of Pharmacy Practice and Center of Excellence for Innovation in Chemistry, Pharmacological Research Unit, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand
› Author AffiliationsSupported by: Naresuan University P2560B037
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Abstract

Reduction of intestinal glucose absorption might result from either delayed carbohydrate digestion or blockage of glucose transporters. Previously, oxyresveratrol was shown to inhibit α-glucosidase, but its effect on glucose transporters has not been explored. The present study aimed to assess oxyresveratrol-induced inhibition of the facilitative glucose transporter 2 and the active sodium-dependent glucose transporter 1. An aqueous extract of Artocarpus lacucha, Puag Haad, which is oxyresveratrol-enriched, was also investigated. Glucose transport was measured by uptake into Caco-2 cells through either glucose transporter 2 or sodium-dependent glucose transporter 1 according to the culture conditions. Oxyresveratrol (40 to 800 µM) dose-dependently reduced glucose transport, which appeared to inhibit both glucose transporter 2 and sodium-dependent glucose transporter 1. Puag Haad at similar concentrations also inhibited these transporters but with greater efficacy. Oxyresveratrol and Puag Haad could help reduce postprandial hyperglycemic peaks, which are considered to be most damaging in diabetics.

Key words

Artocarpus lacucha - Moraceae - oxyresveratrol - glucose transporter - Caco-2 cells

Supporting Information

    Caco-2 cells underwent differentiation within 2 – 3 weeks without special treatment and expressed several intestinal enzymes such as sucrase-isomatase, lactase, and alkaline phosphatase [38]. In the present study, the alkaline phosphatase activity was measured and demonstrated the increase in enzyme activity upon Caco-2 cell differentiation (Fig. 1S). To support the integrity of Caco-2 membrane, in addition to TEER measurement, the characteristics of glucose uptake and absorption were determined (Fig. 2S). The result showed that fluorescence 2-NBDG uptake and direct glucose absorption reached the optimal level at approximately 3 – 4 h; longer incubation did not significantly increase the absorption of glucose, and ~ 10% of glucose was totally absorbed at 24 h. Although these observations were not the direct evidence, they somehow supported the function of cultured Caco-2 membrane.

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Publication History

Received: 06 April 2020

Accepted after revision: 20 November 2020

Article published online:
28 January 2021

© 2021. Thieme. All rights reserved.

Georg Thieme Verlag KG
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