Alkaloid Enantiomers from Isatis tinctoria with Neuroprotective Effects against H2O2-Induced SH-SY5Y Cell Injury

Yu-Fei Xi; Li-Li Lou; Zhi-Yong Xu; Zi-Lin Hou; Xiao-Bo Wang; Xiao-Xiao Huang; Shao-Jiang Song

doi:10.1055/a-1023-8940

Planta Medica, Table of Contents

Planta Med 2019; 85(17): 1374-1382
DOI: 10.1055/a-1023-8940

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Alkaloid Enantiomers from Isatis tinctoria with Neuroprotective Effects against H₂O₂-Induced SH-SY5Y Cell Injury

Yu-Fei Xi

¹School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, Peopleʼs Republic of China

,

Li-Li Lou

¹School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, Peopleʼs Republic of China

,

Zhi-Yong Xu

¹School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, Peopleʼs Republic of China

,

Zi-Lin Hou

¹School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, Peopleʼs Republic of China

,

Xiao-Bo Wang

²Chinese Peopleʼs Liberation Army Logistics Support Force No. 967 Hospital, Dalian, Peopleʼs Republic of China

,

Xiao-Xiao Huang

¹School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, Peopleʼs Republic of China

²Chinese Peopleʼs Liberation Army Logistics Support Force No. 967 Hospital, Dalian, Peopleʼs Republic of China

,

Shao-Jiang Song

¹School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, Peopleʼs Republic of China

› Author Affiliations

Abstract

Six pairs of alkaloid enantiomers including 11 new alkaloids (1a/1b–5a/5b, 6a) were isolated from the leaves of Isatis tinctoria. Their structures were established by extensive spectroscopic analyses. Enantiomers were separated successfully by chiral chromatographic column and the absolute configurations of all isolates were determined by comparison of experimental and calculated electronic circular dichroism spectra. The isolated alkaloids were evaluated for their neuroprotective activities against H₂O₂-induced cell injury in human neuroblastoma SH-SY5Y cells. The results showed that 5a/5b and 6a/6b exhibited potent neuroprotective activities at 50 µM compared with the H₂O₂-treated group.

Key words

Isatis tinctoria - Brassicaceae - alkaloids - enantiomers - neuroprotective effects

Full Text

References

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