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Planta Med 2019; 85(11/12): 911-916
DOI: 10.1055/a-0877-7255
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Development and Validation of a UHPLC-DAD Method for the Quantitative Analysis of Major Dihydrochalcone Glucosides from Thonningia sanguinea VAHL[*]

Luca Pompermaier
1   Institute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Innsbruck, Austria
,
Stefan Schwaiger
1   Institute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Innsbruck, Austria
,
Monizi Mawunu
2   Kimpa Vita University, Province of Uíge, Uíge, Angola
,
Thea Lautenschlaeger
3   Department of Biology, Institute of Botany, Faculty of Science, Technische Universität Dresden, Dresden, Germany
,
Hermann Stuppner
1   Institute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Innsbruck, Austria
› Author Affiliations
Further Information

Publication History

received 30 January 2019
revised 05 March 2019

accepted 11 March 2019

Publication Date:
22 March 2019 (online)

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Abstract

Thonnigia sanguinea is a plant widely used in traditional African medicine against a variety of diseases. The obligate parasite is growing throughout tropical African forests and utilizes a large variety of hosts. Dihydrochalcone glucoside derivatives isolated from the subaerial parts of this plant were identified as potential antidiabetic lead compounds. In this study, an ultrahigh-performance liquid chromatographic method coupled with a photodiode array detector was developed for the quantitation of six major dihydrochalcone derivatives. The analytes were baseline separated in complex samples within 14 minutes on a Phenomenex Luna Omega 1.6 µm C18 column using a mobile phase consisting of water and acetonitrile (each + 0.01% trifluoroacetic acid) in gradient elution. Method validation confirmed the selectivity, linearity (R2 ≥ 0.9992), precision (inter-day ≤ 1.98%, intraday ≤ 2.00%), and accuracy (recovery rates of 97.4 – 106.3% for all analytes). At 280 nm, the LODs and LOQs were found to be lower than 1.42 and 4.30 µg/mL, respectively. Eight plant batches from the northern Angolan province of Uíge (collected in the wild or bought on markets) were extracted with methanol using an ultrasound-assisted extraction protocol and subsequently analyzed with the validated method. Results indicated high contents of dihydrochalcone glucosides in all eight samples. Most notably, the two bioactive constituents thonningianin A and B were present in fairly large amounts (2.42 – 5.35 w%).

Key words

Thonningia sanguinea - Balanophoraceae - UHPLC-DAD - dihydrochalcone glucosides - thonningianin A - thonningianin B

* Dedicated to Professor Dr. Cosimo Pizza in recognition of his outstanding contribution to phytotherapy research.


 

  • References

  • 1 Schmelzer GH, Gurib-Fakim A. Plant Resources of tropical Africa 11 (2): medicinal Plants 2. Wageningen, Netherlands: PROTA Foundation; 2013
    Google Scholar
  • 2 Olanya CA, Eilu G. Host-parasite relations of an angiospermous root parasite (Thonningia sanguinea VAHL) in logged and unlogged sites of Budongo forest reserve, western Uganda. Afr J Ecol 2009; 47: 328-334
    CrossrefPubMedGoogle Scholar
  • 3 Bullock AA. Thonningia VAHL. Kew Bull 1948; 3: 363-367
    CrossrefPubMedGoogle Scholar
  • 4 Neuwinger HD. A Dictionary of Plant Use and Applications with Supplement: Search System for Diseases. Stuttgart: Medpharm Scientific Publishers; 2000
    Google Scholar
  • 5 Iwu MM. Handbook of African medicinal Plants. 2nd ed.. ed. Boca Raton, FL: CRC Press; 2014
    Google Scholar
  • 6 Pompermaier L, Marzocco S, Adesso S, Monizi M, Schwaiger S, Neinhuis C, Stuppner H, Lautenschläger T. Medicinal plants of northern Angola and their anti-inflammatory properties. J Ethnopharmacol 2018; 216: 26-36
    CrossrefPubMedGoogle Scholar
  • 7 Lautenschläger T, Monizi M, Pedro M, Mandombe JL, Bránquima MF, Heinze C, Neinhuis C. First large-scale ethnobotanical survey in the province of Uíge, northern Angola. J Ethnobiol Ethnomed 2018; 14: 1-73
    CrossrefPubMedGoogle Scholar
  • 8 Ohiri FC, Uzodinma VC. Antimicrobial properties of Thonningia sanguinea root extracts. Fitoterapia 2000; 71: 176-178
    CrossrefPubMedGoogle Scholar
  • 9 Gyamfi MA, Aniya Y. Medicinal herb, Thonningia sanguinea protects against aflatoxin B1 acute hepatotoxicity in Fischer 344 rats. Hum Exp Toxicol 1998; 17: 418-423
    CrossrefPubMedGoogle Scholar
  • 10 Gyamfi MA, Hokama N, Oppong-Boachie K, Aniya Y. Inhibitory effects of the medicinal herb, Thonningia sanguinea, on liver drug metabolizing enzymes of rats. Hum Exp Toxicol 2000; 19: 623-631
    CrossrefPubMedGoogle Scholar
  • 11 Nyarko AK, Addy ME. In vitro screening for the anti-anaphylactic agent in Thonningia sanguinea . J Ethnopharmacol 1994; 41: 45-51
    CrossrefPubMedGoogle Scholar
  • 12 Thomford AK, Ahmed Abdelhameed RF, Yamada K. Chemical studies on the parasitic plant Thonningia sanguinea Vahl. RSC Adv 2018; 8: 21002-21011
    CrossrefPubMedGoogle Scholar
  • 13 Pompermaier L, Heiss EH, Alilou M, Mayr F, Monizi M, Lautenschlaeger T, Schuster D, Schwaiger S, Stuppner H. Dihydrochalcone glucosides from the subaerial parts of Thonningia sanguinea and their in vitro PTP1B inhibitory activities. J Nat Prod 2018; 81: 2091-2100
    CrossrefPubMedGoogle Scholar
  • 14 Zhang TT, Yang L, Jiang JG. Effects of thonningianin A in natural foods on apoptosis and cell cycle arrest of HepG-2 human hepatocellular carcinoma cells. Food Funct 2015; 6: 2588-2597
    CrossrefPubMedGoogle Scholar
  • 15 Press MC, Phoenix GK. Impacts of parasitic plants on natural communities. New Phytol 2005; 166: 737-751
    CrossrefPubMedGoogle Scholar
  • 16 Blaschek W, Hilgenfeldt U, Holzgrabe U, Mörike K, Reichling J, Ruth P. HagerROM 2017. Hagers Enzyklopädie der Arzneistoffe und Drogen. Berlin, Heidelberg: Springer; 2017
    Google Scholar
  • 17 International Council for Harmonisation. Validation of analytical procedures: text and methodology Q2 (R1). ICH harmonized tripartite guideline. Available at: https://www.ich.org/products/guidelines/quality/article/quality-guidelines.html Accessed December 28, 2018
    PubMedGoogle Scholar