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Drug Res (Stuttg) 2019; 69(05): 277-283
DOI: 10.1055/a-0664-0431
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Phenyl alanine & Tyrosine Amino acids Coated Magnetic Nanoparticles: Preparation and Toxicity study

Hamed Nosrati
1   Student Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
2   Department of Pharmaceutical Biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
,
Hossein Hamzehei
3   Research laboratory of Advanced Technologies in Medicine, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
,
Saeed Afroogh
4   Department of Medical Biotechnology and Nanotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
,
Seyedeh Fatemeh Ashabi
5   Department of Biology, Faculty of genetics, East Tehran Branch, Islamic Azad University, Tehran, Iran
,
Elahe Attari
6   Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
,
Hamidreza Kheiri Manjili
1   Student Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
6   Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
› Author Affiliations
Further Information

Publication History

received 08 April 2018

accepted 21 July 2018

Publication Date:
06 September 2018 (online)

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Abstract

In this study we reported the synthesis of L-phenyl alanine (Phe) & L-tyrosine (Tyr) Natural Amino acids coated iron oxide magnetic nanoparticles under one-pot and in situ reaction. Functionalized iron oxide magnetic nanoparticles were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Vibrating Sample Magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) techniques. Cellular toxicity of amino acids coated iron oxide magnetic nanoparticles was also investigated on HEK-293 cell lines. Additionally, a hemolysis test of as prepared magnetic nanoparticles were performed. It was found that the synthesized Phe and Tyr coated magnetic nanoparticles (F@Phe NPs and F@Tyr NPs) were spherical in shape with an average size less than 25 nm, also the saturation magnetization (Ms) of the F@Phe NPs and F@Tyr NPs were about 30.02 and 58.23 emu/g, respectively, which was lower than those of bare Fe3O4. The TGA results show that apart from this weight loss, the coated sample shows a weight loss of 5.48, and 6.88% respectively corresponding to loss of Tyr, and Phe which is coated on the Fe3O4 nanoparticles. At a high concentration, less than 2.92 and 3.13% hemolytic activity were observed for F@Phe NPs and F@Tyr NPs, respectively. The F@Phe NPs and F@Tyr NPs show the possibility of using this nanoparticles in the development of in vitro and in vivo pharmaceutical and biomedical fields due to do not possess a toxic effect, good ζ-potential and related small and narrow size distribution.

Key words

Magnetic nanoparticles - Amino acid - Functionalization - Tyrosine - Phenyl alanine - Biocompatibility
 

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