International Journal of Epilepsy 2016; 03(01): 42-62
DOI: 10.1016/j.ijep.2015.12.006
Abstracts
Thieme Medical and Scientific Publishers Private Ltd. 2017

RNA-Seq analysis of hippocampal tissues reveals novel candidate genes for drug refractory epilepsy in patients with MTLE-HS

Aparna Banerjee Dixit
1   Center for Excellence in Epilepsy, A Joint NBRC-AIIMS Collaboration, NBRC, Manesar, India
,
Jyotirmoy Banerjee
1   Center for Excellence in Epilepsy, A Joint NBRC-AIIMS Collaboration, NBRC, Manesar, India
,
Arpna Srivastava
2   Department of Neurosurgery, AIIMS, New Delhi, India
,
Manjari Tripathi
3   Department of Neurology, AIIMS, New Delhi, India
,
Chitra Sarkar
4   Department of Pathology, AIIMS, New Delhi, India
,
Aanchal Kakkar
4   Department of Pathology, AIIMS, New Delhi, India
,
P Sarat Chandra
2   Department of Neurosurgery, AIIMS, New Delhi, India
› Author Affiliations

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Further Information

Publication History

Publication Date:
12 May 2018 (online)

 

    Array-based profiling studies shows aberrant gene expression patterns during epileptogenesis. We have performed RNAseq analysis of the hippocampal tissues resected from the patients with MTLE-HS to investigate the molecular basis of epileptogenicity and/or pharmacoresistance in MTLE. For non-epileptic control experiments, healthy tissues from tumour margins obtained during tumour surgeries were used. RNA sequencing was performed using standard protocols on Illumina HiSeq 2500 platform. Differential gene expression analysis of the RNAseq data revealed 56 significantly regulated genes in MTLE patients and showed that many of these belong to a cohesive network of physically interacting proteins linked to several cellular functions. This study identified various genes like FN1 which is central in our analysis, NEUROD6, RELN, TGF[H9252]R2, NLRP1, SCRT1, CSNK2B, SCN1B, CABP1, KIF5A and antisense RNAs like AQP4-AS1 and KIRREL3-AS2 that needs further evaluation for their potential as diagnostic/prognostic biomarkers in intractable MTLE.


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