Download PDF
Int Arch Otorhinolaryngol 2013; 17(01): 062-065
DOI: 10.7162/S1809-97772013000100011
Original Article
Thieme Publicações Ltda Rio de Janeiro, Brazil

Comparison of fibrin adhesives prepared by 3 different methods

Juliana Benthien Cavichiolo
1   Medical Resident in Otolaryngology, HC-UFPR (Hospital de Clínicas da Universidade Federal do Paraná).
,
Maurício Buschle
2   MMSc. Physician Assistant, Department of Otolaryngology, Faculty of Medicine, UFPR; Associate Professor of Otolaryngology.
,
Bettina Carvalho
3   Otolaryngologist by ABORLCCF.
› Author Affiliations
Further Information

Publication History

01 May 2011

25 October 2012

Publication Date:
06 January 2014 (online)

   Permissions and Reprints

Summary

Introduction: Fibrin tissue adhesive, which has applications in several areas of medicine, can be prepared by different methods.

Aim: To compare fibrin tissue adhesives prepared by 3 different methods.

Method: In this prospective experimental laboratory study, fibrin tissue adhesives prepared by the use of plasma fibrinogen (group 1), cryoprecipitation (group 2), and precipitation by ammonium sulfate (group 3) were tested on 15 rabbits and 10 fragments of dura mater. The quality of the clots was assessed in terms of the success of the healing process, local toxicity, graft adhesion capacity, and degree of adhesion of 2 fragments of dura mater produced.

Results: All methods produced a clot with high adhesion and no toxicity, but tensile strength testing revealed that the glue produced from the ammonium sulfate-precipitated clot (group 3) was the strongest, requiring 39 g/cm2 to separate the fragments as opposed to 23 g/cm2 for group 2 and 13 g/cm2 for group 1.

Conclusion: All methods produced good results as far as clot formation and non-toxicity, but ammonium sulfate precipitation produced the best tensile strength and was thus the most effective method of preparing fibrin tissue adhesive.

Keywords

Fibrin - Fibrin Tissue Adhesive - Biological Therapy - Fibrinogen - Plasma
 

  • References

  • 1 Dohan S, Choukroun J, Dohan A, Donsimoni JM, Gabrieleff D, Fioretti F, Dohan D , Platelet Rich Fibrin (PRF). Un nouveau biomatériau de cicatrisation. Biotechnologies et fibrine, plaquettes et cytokines, aspects immunitaires, implications thérapeutiques. 1re partie: biotechnologies et fibrine. Implantodontie 2004; 13: 87-97
    CrossrefGoogle Scholar
  • 2 Reiss RF, Oz MC. Autologous Fibrin Glue: Production and Clinical Use. Transfusion Medicine Reviews, Vol X, No 2 (April), 1996, 86-92.
    Google Scholar
  • 3 Almeida CIR, Nina LG, Mello RP. Cola biológica autógena. Rev Bras Otorrinolaringol 1991; 57: 115-8
    Google Scholar
  • 4 Bento RF , Miniti. Comparison between fibrin tissue adhesive, epineural suture and natural union in intratemporal facial nerve of cats. Acta Oto-Laryngologica 1989; 465: 1-36
    Google Scholar
  • 5 Jessen C, Sharma P. Use of fibrin glue in thoracic surgery. The Annals os Thoracic Surgery 1985; 39 (6) 521-4
    Google Scholar
  • 6 Tidrick RT, Seegers WH, Warner ED. Clinical experience with thrombin as na hemostatic agent. Surgery 1943; 14: 191-6
    Google Scholar
  • 7 Bento RF, Salomone R, Tsuji RK, Housen M, Neto RB. O Uso de Cola de Fibrina Humana na Anastomose de Lesões Traumáticas Parciais do Nervo Facial. Arq. Int. Otorrinolaringol 2008; 12 (2) 214-219
    Google Scholar
  • 8 Sterkers O, Becherel P, Sterkers JM. Reparation du nerf facial par colle de fibrine exclusive 56 cas. Annals Otolaryngolo ic Chirurgical Cervicofacial 1989; 106: 176-81
    Google Scholar
  • 9 Siedentop KH, Harris DM, Sanchez B. Autologous fibrin tissue. Laryngoscope 1985; 9: 1074-76
    Google Scholar