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CC BY-NC-ND 4.0 · Libyan International Medical University Journal 2021; 06(02): 75-80
DOI: 10.4103/liuj.liuj_79_21
Original Article

Characterization and antibiotic resistance of staphylococci strains isolated from brack hospital in the Southern Region of Libya

Ghada Alshaibani
Department of Life Sciences, School of Basic Sciences, Libyan Academy of Postgraduate Studies, Tripoli
,
Aisha Shahlol
1   Department of Medical Laboratory Technology, Faculty of Engineering and Technology, Sabha University, Sabha, Libya
,
Ahmed Abid
2   National Centre for Disease Control, Tripoli
,
Samira Amri
3   Burns and Plastic Surgery Centre, Tripoli
,
Ezzeddin Aghila
3   Burns and Plastic Surgery Centre, Tripoli
,
Ghaith Abdalah
3   Burns and Plastic Surgery Centre, Tripoli
,
Mohamed Ahmed
4   Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, Tripoli
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Abstract

Background: Staphylococcus species are one of the most important healthcare-associated pathogens able to acquire and spread multidrug-resistant determinants. Objectives: The present study investigated and analyzed 110 potential Staphylococcus species isolated from various clinical and nonclinical samples at Brack hospital. Materials and Methods: A nonselective laboratory approach was applied, using cultural characteristics, Gram stain, and catalase reactivity followed by confirmation at the species levels and determination the susceptibility against antimicrobial agents using the Phoenix automated microbiological system. Results: In total, 57.5% were confirmed as species and subspecies of Staphylococcus represented by ten different species: nine subspecies of coagulase-negative staphylococci (CoNS) (76.2%) and one coagulase-positive staphylococci (CoNS) subspecies (23.8%). Of these strains, 16.6% were identified as methicillin-resistant staphylococci (MRS) mostly of the CoNS group expressing significant resistance to important antimicrobial classes. Conclusion: This study reports a high prevalence of various staphylococci species, particularly of CoNS group expressing multidrug resistance patterns of public health concern, from a healthcare setting in the south region of Libya. The identification of higher rate of MRCoNS underlines the importance of monitoring all multidrug-resistant staphylococci species requiring further epidemiological investigations.

Key-words:

Antimicrobial susceptibility testing - Brack hospital - coagulase-negative staphylococci - Libya - multidrug-resistant staphylococci

Financial support and sponsorship

Nil.




Publication History

Received: 30 June 2021

Accepted: 12 August 2021

Article published online:
14 June 2022

© 2021. Libyan International Medical University. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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  • References

  • 1 World Health Organization. Critically Important Antimicrobials for Human Medicine. 5th Revision; 2017. https://www.who.int/foodsafety/publications/cia2017.pdf. [Las accessed on 2019 Jul 30].
  • 2 Littmann J, Viens AM. The ethical significance of antimicrobial resistance. Public Health Ethics 2015;8:209-24.
  • 3 Ahmed MO, Baptiste KE, Daw MA, Elramalli AK, Abouzeed YM, Petersen A. Spa typing and identification of pvl genes of meticillin-resistant Staphylococcus aureus isolated from a Libyan hospital in Tripoli. J Glob Antimicrob Resist 2017;10:179-81.
  • 4 Ahmed MO, Elramalli AK, Baptiste KE, Daw MA, Zorgani A, Brouwer E, et al. Whole Genome Sequence Analysis of the First Vancomycin-Resistant Enterococcus faecium Isolates from a Libyan Hospital in Tripoli. Microb Drug Resist 2020;26:1390-8.
  • 5 Ahmad KM, Alamen AA, Atiya FA, Elzen AA. Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) among Staphylococcus aureus collection at Sebha medical center. J Adv Lab Res Biol 2018;9:01-8.
  • 6 Ahmad KM, Alamen AA, Shamsi SA, Elzen AA. Co-expression of extended spectrum β-lactamase (ESBL) and AmpC β-lactamase among Pseudomonas aeruginosa clinical isolates in sebha medical center. J Pure Appl Sci 2019;18:11-6.
  • 7 Elnageh HR, Hiblu MA, Abbassi MS, Abouzeed YM, Ahmed MO. Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs. Open Vet J 2021;10:452-6.
  • 8 Othman AA, Hiblu MA, Abbassi MS, Abouzeed YM, Ahmed MO. Nasal colonization and antibiotic resistance patterns of Staphylococcus species isolated from healthy horses in Tripoli, Libya. J Equine Sci 2021;32:61-5.
  • 9 Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. Wayne, Pennsylvania: CLSI Supplement M100; 2020.
  • 10 Aetrugh SM, Aboshkiwa MA, Husien WM, Erhuma ME, Corrente M, Grandolfo E, et al. Antimicrobial resistance profile and molecular characterization of methicillin-resistant staphylococcus isolates in Tripoli Central Hospital, Libya. Libyan Int Med Univ J 2017;2:74-83.
  • 11 Al-Abdli NE, Baiu SH. Isolation of MRSA Strains from hospital environment in Benghazi city, Libya. Am J Inf Dis Microbiol 2016;4:41-3.
  • 12 Otto M. Staphylococcus epidermidis--the 'accidental' pathogen. Nat Rev Microbiol 2009;7:555-67.
  • 13 Schilcher K, Horswill AR. Staphylococcal biofilm development: Structure, regulation, and treatment strategies. Microbiol Mol Biol Rev 2020;84:e00026-19.
  • 14 Becker K, Heilmann C, Peters G. Coagulase-negative staphylococci. Clin Microbiol Rev 2014;27:870-926.
  • 15 Mendoza-Olazarán S, Morfin-Otero R, Rodríguez-Noriega E, Llaca-Díaz J, Flores-Treviño S, González-González GM, et al. Microbiological and molecular characterization of Staphylococcus hominis isolates from blood. PLoS One 2013;8:e61161.
  • 16 Nobrega DB, Naushad S, Naqvi SA, Condas LA, Saini V, Kastelic JP, et al. Prevalence and genetic basis of antimicrobial resistance in non-aureus staphylococci isolated from Canadian dairy herds. Front Microbiol 2018;9:256.
  • 17 Velasco V, Buyukcangaz E, Sherwood JS, Stepan RM, Koslofsky RJ, Logue CM. Characterization of Staphylococcus aureus from Humans and a comparison with İsolates of animal origin, in north Dakota, United States. PLoS One 2015;10:e0140497.
  • 18 Giordano N, Corallo C, Miracco C, Papakostas P, Montella A, Figura N, et al. Erythema nodosum associated with Staphylococcus xylosus septicemia. J Microbiol Immunol Infect 2016;49:134-7.
  • 19 Leroy S, Vermassen A, Ras G, Talon R. Insight into the genome of Staphylococcus xylosus, a ubiquitous species well adapted to meat products. Microorganisms 2017;5:E52.
  • 20 Bal EB, Bayar S, Bal MA. Antimicrobial susceptibilities of coagulase-negative staphylococci (CNS) and streptococci from bovine subclinical mastitis cases. J Microbiol 2010;48:267-74.
  • 21 Lakhundi S, Zhang K. Methicillin-resistant Staphylococcus aureus: Molecular characterization, evolution, and epidemiology. Clin Microbiol Rev 2018;31:e00020-18.
  • 22 Venugopal N, Mitra S, Tewari R, Ganaie F, Shome R, Rahman H, et al. Molecular detection and typing of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci isolated from cattle, animal handlers, and their environment from Karnataka, Southern Province of India. Vet World 2019;12:1760-8.
  • 23 Morgenstern M, Erichsen C, Hackl S, Mily J, Militz M, Friederichs J, et al. Antibiotic resistance of commensal Staphylococcus aureus and coagulase-negative Staphylococci in an International cohort of surgeons: A prospective point-prevalence study. PLoS One 2016;11:e0148437.
  • 24 Han JI, Yang CH, Park HM. Prevalence and risk factors of Staphylococcus spp. carriage among dogs and their owners: A cross-sectional study. Vet J 2016;212:15-21.
  • 25 Kateete DP, Asiimwe BB, Mayanja R, Mujuni B, Bwanga F, Najjuka CF, et al. Nasopharyngeal carriage, spa types and antibiotic susceptibility profiles of Staphylococcus aureus from healthy children less than 5 years in Eastern Uganda. BMC Infect Dis 2019;19:1023.
  • 26 Xu Z, Shah HN, Misra R, Chen J, Zhang W, Liu Y, et al. The prevalence, antibiotic resistance and mecA characterization of coagulase negative staphylococci recovered from non-healthcare settings in London, UK. Antimicrob Resist Infect Control 2018;7:73.
  • 27 Bietrix J, Kolenda C, Sapin A, Haenni M, Madec JY, Bes M, et al. Persistence and Diffusion of mecC-positive CC130 MRSA isolates in dairy farms in Meurthe-et-Moselle county (France). Front Microbiol 2019;10:47.
  • 28 McGuinness WA, Malachowa N, DeLeo FR. Vancomycin resistance in Staphylococcus aureus. Yale J Biol Med 2017;90:269-81.
  • 29 Gómez-Sanz E, Ceballos S, Ruiz-Ripa L, Zarazaga M, Torres C. clonally diverse Methicillin and multidrug resistant coagulase negative staphylococci are ubiquitous and pose transfer ability between pets and their owners. Front Microbiol 2019;10:485.
  • 30 Sunagar R, Hegde NR, Archana GJ, Sinha AY, Nagamani K, Isloor S. Prevalence and genotype distribution of methicillin-resistant Staphylococcus aureus (MRSA) in India. J Glob Antimicrob Resist 2016;7:46-52.
  • 31 Carroll KC, Borek AP, Burger C, Glanz B, Bhally H, Henciak S, et al. Evaluation of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of staphylococci and enterococci. J Clin Microbiol 2006;44:2072-7.
  • 32 Chatzigeorgiou KS, Sergentanis TN, Tsiodras S, Hamodrakas SJ, Bagos PG. Phoenix100 versus Vitek 2 in the identification of gram-positive and gram-negative bacteria: A comprehensive meta-analysis. J Clin Microbiol 2011;49:3284-91.
  • 33 Guardabassi L, Damborg P, Stamm I, Kopp PA, Broens EM, Toutain PL, et al. Diagnostic microbiology in veterinary dermatology: Present and future. Vet Dermatol 2017;28:146-e30.