CC BY-NC-ND 4.0 · Indian J Med Paediatr Oncol 2020; 41(04): 519-522
DOI: 10.4103/ijmpo.ijmpo_248_19
Original Article

R248W Mutations in p53 Gene are Rare Among Indian Patients with Head-and-Neck Cancer

Ashna Gauthaman
Department of Integrative Biology, School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India
,
Anbalagan Moorthy
Department of Integrative Biology, School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India
› Author Affiliations
Financial support and sponsorship This study was financially supported by Vellore Institute of Technology – VIT seed grant (12927).

Abstract

Aim

Cancer is one of the curses to humankind, decades of research in eradicating the disease from the society is proven difficult. Close interaction between clinicians and scientists helps us to translate clinical observations into molecular mechanism of the disease. The Cancer Genome Atlas data suggest that genetic alterations in p53 gene play a crucial role in head-and-neck squamous cell carcinoma (HNSCC) tumorigenesis. Understanding p53 aberrations and their impact on other cellular activities can help with the design of new, more effective therapeutic strategy that target p53 mutation-bearing HNSCC, thereby producing a personalized medicine approach for the disease.

Materials and Methods

In an effort to identify the role of R248W mutation of p53 gene in HNSCC patients of Indian origin, tumor samples were collected from 55 patients (n = 55), and polymerase chain reaction–restriction fragment length polymorphism technique was used to screen for the mutation using genomic DNA isolated from the tumors.

Results

The results reveal that except for one patient (heterozygous), all the patients were negative for the mutation.

Conclusion

These results suggest that p53 R248W mutations are less prevalent in HNSCC Indian patients.



Publication History

Received: 13 December 2019

Accepted: 29 June 2020

Article published online:
17 May 2021

© 2020. Indian Society of Medical and Paediatric Oncology. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/.)

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