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CC BY-NC-ND 4.0 · J Lab Physicians 2019; 11(01): 017-022
DOI: 10.4103/JLP.JLP_98_18
Original Article

Detection of biofilm among uropathogenic Escherichia coli and its correlation with antibiotic resistance pattern

Rashmi M. Karigoudar
Department of Microbiology, BLDE (Deemed to be University) Shri B M Patil Medical College, Vijayapura, Karnataka, India
,
Mahesh H. Karigoudar
Department of Pathology, BLDE (Deemed to be University) Shri B M Patil Medical College, Vijayapura, Karnataka, India
,
Sanjay M. Wavare
Department of Microbiology, BLDE (Deemed to be University) Shri B M Patil Medical College, Vijayapura, Karnataka, India
,
Smita S. Mangalgi
Department of Microbiology, BLDE (Deemed to be University) Shri B M Patil Medical College, Vijayapura, Karnataka, India
› Author Affiliations
Financial support and sponsorship: Nil
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Abstract

BACKGROUND: Escherichia coli accounts for 70%–95% of urinary tract infections (UTIs). UTI is a serious health problem with respect to antibiotic resistance and biofilms formation being the prime cause for the antibiotic resistance. Biofilm can restrict the diffusion of substances and binding of antimicrobials. In this context, the present study is aimed to perform in vitro detection of biofilm formation among E. coli strains isolated from urine and to correlate their susceptibility pattern with biofilm formation.

MATERIALS AND METHODS: A total of 100 E. coli strains isolated from patients suffering from UTI were included in the study. The identification of E. coli was performed by colony morphology, Gram staining, and standard biochemical tests. The detection of biofilm was carried out by Congo Red Agar (CRA) method, tube method (TM), and tissue culture plate (TCP) method. Antimicrobial sensitivity testing was performed by Kirby–Bauer disc diffusion method on Muller–Hinton agar plate.

RESULTS: Of the 100 E. coli strains, 49 (49%) and 51 (51%) were from catheterized and noncatheterized patients, respectively. Biofilm production was positive by CRA, TM, and TCP method were 49 (49%), 55 (55%), and 69 (69%), respectively. Biofilm producers showed maximum resistance to co-trimoxazole (73.9%), gentamicin (94.2%), and imipenem (11.6%) when compared to nonbiofilm producers. Significant association was seen between resistance to antibiotic and biofilm formation with a P = 0.01 (<0.05).

CONCLUSION: A greater understanding of biofilm detection in E. coli will help in the development of newer and more effective treatment. The detection of biofilm formation and antibiotic susceptibility pattern helps in choosing the correct antibiotic therapy.

Key words

Antibiotic resistance - biofilm - Escherichia coli


Publication History

Received: 24 July 2018

Accepted: 17 December 2018

Article published online:
06 April 2020

© 2019.

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

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