PDF herunterladen
Open Access
CC BY-NC-ND 4.0 · J Lab Physicians 2019; 11(04): 305-311
DOI: 10.4103/JLP.JLP_59_19
Original Article

Co occurrence of two 16S rRNA methyltrasferases along with NDM and OXA 48 like carbapenamases on a single plasmid in Klebsiella pneumoniae

Authors

  • K. V. L. Aishwarya

    Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India

  • P. V. Geetha

    Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India

  • M. Shanthi

    Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India

  • S. Uma

    Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India

Financial support and sponsorship This study was supported by the Department of Science and Technology under INSPIRE-fellowship scheme (reference number IF150368).
Weitere Informationen
Auch verfügbar auf
 als PDF herunterladen Lizenzen und Reprints
Preview

Abstract

BACKGROUND: The carbapenemase-encoding genes, bla NDM- and bla OXA-48 - like , confer resistance to all the known beta-lactams and are encountered along with other beta-lactamase-encoding genes and/or 16S ribosomal RNA (rRNA)-methylating genes. The co-occurrence of bla NDM and bla OXA-48 - like on a single plasmid is a rare occurrence.

AIM AND OBJECTIVE: The purpose of the study was to characterize the plasmids in Klebsiella pneumoniae isolates producing 16S rRNA methyltransferase along with bla NDM , bla OXA-48-like , and other resistance encoding genes.

MATERIALS AND METHODS: One-hundred and seventeen K. pneumoniae clinical isolates which were resistant to aminoglycosides were collected. Polymerase chain reaction-based screening for 16S rRNA methyltransferase genes armA, rmtB, and rmtC; carbapenamase genes bla NDM , bla OXA-48-like , bla IMP, bla VIM, and bla KPC ; and other resistance genes such as bla TEM, bla SHV, bla CTX-M , and qnr (A, B, and S) determinants acc (6') Ib-cr was performed. Conjugation experiment was carried out for seven isolates that anchored bla NDM and bla OXA-48-like along with any one of the 16S rRNA methyltransferases. The plasmid-based replicon typing for different plasmid-incompatible (Inc) group was performed on the conjugatively transferable plasmids.

RESULTS: Among the 16S rRNA methyltransferases, armA was more predominant. bla NDM and bla OXA-48 -like were present in 56 (47.86%) and 22 (18.80%) isolates, respectively. Out of seven isolates which were conjugatively transferable, only four had bla NDM and bla OXA-48 - like on the same plasmid and they belonged to Inc N and A/C replicon. Three isolates co-harbored 16S rRNA methyltransferases armA, rmtB, and rmtC, and out of the them, one isolate harbored two 16S rRNA methyltransferases armA and rmtB, on the single-plasmid replicon A/C.

CONCLUSION: This is the first report revealing the coexistence of bla NDM and bla OXA-48 - like co-harboring two 16S rRNA methylases on a single conjugative plasmid replicon belonging to incompatibility group A/C.

Key words

16S ribosomal RNA methyltransferase - A/C replicon - bla NDM - bla OXA-48 like - co-harboring - Klebsiella pneumoniae


Publikationsverlauf

Eingereicht: 08. April 2019

Angenommen: 07. November 2019

Artikel online veröffentlicht:
07. April 2020

© 2019.

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • References

  • 1 Yokoyama K, Doi Y, Yamane K, Kurokawa H, Shibata N, Shibayama K, et al. Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa. Lancet 2003;362:1888-93.
  • 2 Galimand M, Courvalin P, Lambert T. Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation. Antimicrob Agents Chemother 2003;47:2565-71.
  • 3 Rahman M, Prasad KN, Pathak A, Pati BK, Singh A, Ovejero CM, et al. RmtC and rmtF 16S rRNA methyltransferase in NDM-1-producing Pseudomonas aeruginosa. Emerg Infect Dis 2015;21:2059-62.
  • 4 Ayad A, Drissi M, de Curraize C, Dupont C, Hartmann A, Solanas S, et al. Occurrence of armA and rmtB aminoglycoside resistance 16S rRNA methylases in extended-spectrum β-lactamases producing Escherichia coli in Algerian hospitals. Front Microbiol 2016;7:1409.
  • 5 Wangkheimayum J, Paul D, Dhar D, Nepram R, Chetri S, Bhowmik D, et al. Occurrence of acquired 16S rRNA methyltransferase-mediated aminoglycoside resistance in clinical isolates of Enterobacteriaceae within a tertiary referral hospital of Northeast India. Antimicrob Agents Chemother 2017;61:e01037-16.
  • 6 Berçot B, Poirel L, Nordmann P. Updated multiplex polymerase chain reaction for detection of 16S rRNA methylases: High prevalence among NDM-1 producers. Diagn Microbiol Infect Dis 2011;71:442-5.
  • 7 Bakour S, Alsharapy SA, Touati A, Rolain JM. Characterization of Acinetobacter baumannii clinical isolates carrying bla (OXA-23) carbapenemase and 16S rRNA methylase ArmA genes in Yemen. Microb Drug Resist 2014;20:604-9.
  • 8 Karah N, Haldorsen B, Hermansen NO, Tveten Y, Ragnhildstveit E, Skutlaberg DH, et al. Emergence of OXA-carbapenemase – And 16S rRNA methylase-producing international clones of Acinetobacter baumannii in Norway. J Med Microbiol 2011;60:515-21.
  • 9 Balm MN, La MV, Krishnan P, Jureen R, Lin RT, Teo JW. Emergence of Klebsiella pneumoniae co-producing NDM-type and OXA-181 carbapenemases. Clin Microbiol Infect 2013;19:E421-3.
  • 10 Gokmen TG, Nagiyev T, Meral M, Onlen C, Heydari F, Koksal F. NDM-1 and rmtC-producing Klebsiella pneumoniae isolates in Turkey. Jundishapur J Microbiol 2016;9:e33990.
  • 11 Poirel L, Dortet L, Bernabeu S, Nordmann P. Genetic features of blaNDM-1-positive Enterobacteriaceae. Antimicrob Agents Chemother 2011;55:5403-7.
  • 12 Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods 2005;63:219-28.
  • 13 Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing. CLSI Supplement M100S. CLSI Supplement M100S. Wayne, PA: Clinical and Laboratory Standards Institute; 2016.
  • 14 Yan JJ, Wu JJ, Ko WC, Tsai SH, Chuang CL, Wu HM, et al. Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals. J Antimicrob Chemother 2004;54:1007-12.
  • 15 Doi Y, Arakawa Y. 16S ribosomal RNA methylation: Emerging resistance mechanism against aminoglycosides. Clin Infect Dis 2007;45:88-94.
  • 16 Nordmann P, Naas T, Poirel L. Global spread of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 2011;17:1791-8.
  • 17 Hassan MI, Alkharsah KR, Alzahrani AJ, Obeid OE, Khamis AH, Diab A. Detection of extended spectrum beta-lactamases-producing isolates and effect of AmpC overlapping. J Infect Dev Ctries 2013;7:618-29.
  • 18 Robicsek A, Strahilevitz J, Sahm DF, Jacoby GA, Hooper DC. Qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States. Antimicrob Agents Chemother 2006;50:2872-4.
  • 19 Wareham DW, Umoren I, Khanna P, Gordon NC. Allele-specific polymerase chain reaction (PCR) for rapid detection of the aac (6')-Ib-cr quinolone resistance gene. Int J Antimicrob Agents 2010;36:476-7.
  • 20 Potron A, Poirel L, Nordmann P. Plasmid-mediated transfer of the bla (NDM-1) gene in gram-negative rods. FEMS Microbiol Lett 2011;324:111-6.
  • 21 Wang H, Chen M, Ni Y, Liu Y, Sun H, Yu Y, et al. Antimicrobial resistance among clinical isolates from the Chinese meropenem surveillance study (CMSS), 2003-2008. Int J Antimicrob Agents 2010;35:227-34.
  • 22 Wachino J, Arakawa Y. Exogenously acquired 16S rRNA methylases found in aminoglycoside- resistant pathogenic gram negative bacteria: An update. Drug Resist Updat 2012;15:133-48.
  • 23 Yang J, Ye L, Wang W, Luo Y, Zhang Y, Han L, et al. Diverse prevalence of 16S rRNA methylase genes armA and rmtB amongst clinical multidrug-resistant Escherichia coli and Klebsiella pneumoniae isolates. Int J Antimicrob Agents 2011;38:348-51.
  • 24 Manoharan A, Premalatha K, Chatterjee S, Mathai D; SARI Study Group. Correlation of TEM, SHV and CTX-M extended-spectrum beta lactamases among Enterobacteriaceae with theirin vitro antimicrobial susceptibility. Indian J Med Microbiol 2011;29:161-4.
  • 25 Goyal A, Prasad KN, Prasad A, Gupta S, Ghoshal U, Ayyagari A. Extended spectrum beta-lactamases in Escherichia coli and Klebsiella pneumoniae and associated risk factors. Indian J Med Res 2009;129:695-700.
  • 26 Veeraraghavan B, Shankar C, Karunasree S, Kumari S, Ravi R, Ralph R. Carbapenem resistant Klebsiella pneumoniae isolated from bloodstream infection: Indian experience. Pathog Glob Health 2017;111:240-6.
  • 27 Galimand M, Sabtcheva S, Courvalin P, Lambert T. Worldwide disseminated armA aminoglycoside resistance methylase gene is borne by composite transposon Tn1548. Antimicrob Agents Chemother 2005;49:2949-53.
  • 28 Barguigua A, Zerouali K, Katfy K, El Otmani F, Timinouni M, Elmdaghri N, et al. Occurrence of OXA-48 and NDM-1 carbapenemase-producing Klebsiella pneumoniae in a Moroccan University hospital in Casablanca, Morocco. Infect Genet Evol 2015;31:142-8.
  • 29 Soundari PG, Vijyakumar R, Krishnan P. Abstracts of the 27th European Congress of Clinical Microbiology and Infectious Diseases. Vienna, Austria; 2017.
  • 30 Poirel L, Lagrutta E, Taylor P, Pham J, Nordmann P. Emergence of metallo-β-lactamase NDM-1-producing multidrug-resistant Escherichia coli in Australia. Antimicrob Agents Chemother 2010;54:4914-6.