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CC BY-NC-ND 4.0 · J Lab Physicians 2018; 10(04): 414-419
DOI: 10.4103/JLP.JLP_55_18
Original Article

Characterization and antimicrobial susceptibility of coagulase-negative staphylococci isolated from clinical samples

Prapti Bora
Department of Microbiology, Government Medical College and Hospital, Chandigarh, India
,
Priya Datta
Department of Microbiology, Government Medical College and Hospital, Chandigarh, India
,
Varsha Gupta
Department of Microbiology, Government Medical College and Hospital, Chandigarh, India
,
Lipika Singhal
Department of Microbiology, Government Medical College and Hospital, Chandigarh, India
,
Jagdish Chander
Department of Microbiology, Government Medical College and Hospital, Chandigarh, India
› Author Affiliations Financial support and sponsorship: Nil.
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ABSTRACT

PURPOSE: This study has been done to speciate coagulase-negative staphylococci (CoNS) and also study their antibiotic susceptibility pattern isolated from clinical samples.

MATERIALS AND METHODS: A total of 120 consecutive CoNS were isolated from various clinical samples such as blood, pus, wound swab, drain fluid, tracheal aspirate, peritoneal fluid, and pleural fluid over a period of 6 months. CoNS were identified by characteristic growth on media such as Blood agar and MacConkey agar. Speciation and identification were done by a range of biochemical testing such as PYR broth hydrolysis, novobiocin resistance, polymyxin B sensitivity, and then by matrix-assisted laser desorption ionization-time of flight. Antibiotic susceptibility of the isolates was done by Kirby-Bauer disk diffusion method as per CLSI 2017 guidelines.

RESULTS: Among the 120 isolates, the most common species was Staphylococcus epidermidis (56.67%) followed by Staphylococcus haemolyticus (21.67%), Staphylococcus lugdunensis (11.67%), Staphylococcus caprae (5%), Staphylococcus cohnii (3.33%), and finally Staphylococcus vitulinus (1.67%). Good in vitro susceptibility was noted toward linezolid (100%), vancomycin (100%), teicoplanin (100%), and doxycycline (80.2%). The antibiotics to which resistance was seen were penicillin (96.5%), ciprofloxacin (57.1%), and oxacillin (45.5%). MR CoNS in our study ranged from 50% to 68.67%.

CONCLUSION: Antibiotic resistance in CoNS is increasing toward penicillin, ciprofloxacin, and oxacillin as found in our study. The antibiotics such as vancomycin, teicoplanin, linezolid, and doxycycline which showed good in vitro susceptibility, therefore, should be kept as reserve drugs and used judiciously.

Keywords

Antibiotic susceptibility - coagulase negative staphylococci - iMLSB


Publication History

Received: 21 April 2018

Accepted: 14 August 2018

Article published online:
20 February 2020

© 2018.

Thieme Medical and Scientific Publishers Private Ltd.
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