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CC BY-NC-ND 4.0 · J Lab Physicians 2018; 10(02): 189-193
DOI: 10.4103/JLP.JLP_111_17
Original Article

Alternative to xylene as a clearing agent in histopathology

Nasar Alwahaibi
Department of Allied Health Sciences, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman
,
Shaima Aljaradi
Department of Pathology, Sultan Qaboos University Hospital, Muscat, Oman
,
Horiyah Alazri
Department of Pathology, Sultan Qaboos University Hospital, Muscat, Oman
› Institutsangaben Financial support and sponsorship Nil.
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ABSTRACT

INTRODUCTION: Clearing is an essential step in processing tissue for light microscopy. Xylene is the clearing agent used most commonly worldwide. Xylene is toxic and therefore a threat to personnel working in histopathology laboratories. We evaluated a safer alternative clearing agent for use in the histopathology laboratory.

MATERIALS AND METHODS: We used 230 formalin-fixed, paraffin-embedded tissue blocks from 19 different tissues. Half of the specimens were processed using xylene and half were processed using UltraClear™. Tissues were evaluated for eight parameters: sectioning, nuclear staining, cytoplasmic staining, overall cell morphology, clarity of staining, uniformity of staining, quality of immunohistochemistry (IHC), and cost.

RESULTS: Both UltraClear™ and xylene processed sections scored 100% for IHC. Sections processed using UltraClear™ were easy to cut (81.7%) as were xylene processed sections (96.5%). UltraClear™ processed sections showed 67%, 60.9%, 52.2%, 63.5%, and 67% for nuclear staining, cytoplasmic staining, cell morphology, clarity of staining, and uniformity of staining, respectively. UltraClear™ is twice as expensive as xylene. We found that tissues processed using UltraClear™ were easy to cut and worked well for both hematoxylin and eosin and IHC staining.

CONCLUSION: UltraClear™ is less toxic, less flammable, friendlier to the environment, and easy to handle, but it is two times expensive than xylene. The findings of this study recommend the use of UltraClear™ solution as a routine clearing agent in histopathology laboratories. However, further studies are required.

Keywords

Clearing agent - histopathology - histoprocessing - UltraClear™ - xylene


Publikationsverlauf

Eingereicht: 13. Juni 2017

Angenommen: 09. Juli 2017

Artikel online veröffentlicht:
19. Februar 2020

© 2018.

Thieme Medical and Scientific Publishers Private Ltd.
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  • REFERENCES

  • 1 Metgud R, Astekar MS, Soni A, Naik S, Vanishree M. Conventional xylene and xylene-free methods for routine histopathological preparation of tissue sections. Biotech Histochem 2013;88:235-41.
  • 2 Pandey P, Dixit A, Tanwar A, Sharma A, Mittal S. A comparative study to evaluate liquid dish washing soap as an alternative to xylene and alcohol in deparaffinization and hematoxylin and eosin staining. J Lab Physicians 2014;6:84-90.
  • 3 Udonkang M, Eluwa M, Ekanem A, Sharma TB, Asuquo OR, Akpantah AO. Bleached palm oil as substitute for xylene in histology. J Pharm Clin Res 2014;8:8-17.
  • 4 Rajan ST, Malathi N. Health hazards of xylene: A literature review. J Clin Diagn Res 2014;8:271-4.
  • 5 Material Safety Data Sheet (MSDS) for Xylene Fisher Scientific; 2008. Available from: https://www.fscimage.fishersci.com/msds/25150.htm. [Last accessed on 2017 Mar 22].
  • 6 Sermadi W, Prabhu S, Acharya S, Javali SB. Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory. J Oral Maxillofac Pathol 2014;18:49-53.
  • 7 Material Safety Data Sheet (MSDS) for UltraClear™ Avantor Performance Materials B.V; 2010. Available from: http://www.avantormaterials.com/documents/MSDS/DEV/GB/MSDS_3905_GB.pdf. [Last accessed on 2017 Mar 22].
  • 8 Bancroft JD, Gamble M. Theory and Practice of Histological Techniques. 6th ed. Philadelphia, USA: Churchill Livingstone Elsevier; 2008. p. 126-7.
  • 9 Singh A, Hunasgi S, Koneru A, Vanishree M, Ramalu S, Manvikar V, et al. Comparison of honey with ethanol as an oral cytological fixative: A pilot study. J Cytol 2015;32:113-7.
  • 10 Buesa RJ. Mineral oil: The best xylene substitute for tissue processing yet? J Histotechnol 2000;23:143-9.
  • 11 Kunhua W, Chuming F, Tao L, Yanmei Y, Xin Y, Xiaoming Z, et al. Anovel non-toxic xylene substitute (SBO) for histology. Afr J Tradit Complement Altern Med 2012;9:43-9.
  • 12 Aydin I, Yörükoglu K, Cingöz S, Agilkaya S. The effect of the alternative solutions to formaldehyde and xylene on tissue processing. Indian J Pathol Microbiol 2013;56:221-30.
  • 13 Stockert JC, López-Arias B, Del Castillo P, Romero A, Blázquez-Castro A. Replacing xylene with n-heptane for paraffin embedding. Biotech Histochem 2012;87:464-7.
  • 14 Swamy SR, Nandan SR, Kulkarni PG, Rao TM, Palakurthy P. Bio-friendly alternatives for xylene – Carrot oil, olive oil, pine oil, rose oil. J Clin Diagn Res 2015;9:ZC16-8.
  • 15 Adeniyi IM, Adejoba OR, Akinlabi FM, Alao OJ. Vegetable oils as clearing agents. Achieve Life Sci 2016;10:1-4.