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CC BY-NC-ND 4.0 · European Journal of General Dentistry 2015; 4(01): 3-7
DOI: 10.4103/2278-9626.149669
Original Article

Bacterial microleakage of aged adhesive restorations

Nevin Cobanoglu
Department of Restorative Dentistry, Faculty of Dentistry, University of Selcuk, Konya, Turkey
,
Emine Kara
1   Department of Restorative Dentistry, Faculty of Dentistry, University of Yüzüncü Yil, Van, Turkey
,
Nimet Unlu
2   Department of Restorative Dentistry, Faculty of Dentistry, University of Necmettin Erbakan, Konya, Turkey
,
Fusun Ozer
3   Department of Preventive and Restorative Sciences, University of Pennsylvania, Philadelphia, USA
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Abstract

Objective: The aim of this study was to investigate the marginal bacterial leakage of two self-etch adhesive systems after long-term water storage. Materials and Methods: Class V cavities were prepared on the buccal and lingual surfaces of extracted premolar teeth. After the sterilization of the teeth, four cavities were not restored for control purposes, whereas the other teeth were divided into two groups (n = 16 cavities each): Clearfil Protect Bond (CPB), Clearfil SE Bond (CSE). After the application of the bonding agent, cavities were restored with a composite resin. Then, the teeth were thermo cycled, stored in saline solution for 6 months and put into a broth culture of Streptococcus mutans. The teeth were fixed, sectioned and stained using the Gram-Colour modified method. The stained sections were then evaluated under a light microscope. The bacterial leakage was scored as: 0 - absence of stained bacteria, 1 - bacterial staining along the cavity walls, 2 - bacterial staining within the cut dentinal tubules. The data were analysed using the Kruskal—Wallis and Mann—Whitney U-test (P = 0.05). Results: The bacterial staining was detected within the cut dentinal tubules in all control cavities, in three cavities in the CSE group and one cavity in the CPB group. There were no observed statistically significant differences between the bacterial penetrations of the two bonding systems (P > 0.05). Conclusion: Both bonding systems provided acceptable prevention of marginal bacterial leakage after long-term water storage.

Keywords

Antibacterial adhesive - bacterial microleakage - self-etching adhesive


Publication History

Article published online:
01 November 2021

© 2015. European Journal of General Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/.)

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