CC BY-NC-ND 4.0 · European Journal of General Dentistry 2014; 3(01): 57-65
DOI: 10.4103/2278-9626.126214
Original Article

Insights into and relative effect of chitosan-krill oil, chitosan-H-aspirin, chitosan-H-krill oil-nystatin and chitosan-H-krill oil-aspirin-nystatin on dentin bond strength and functional drug delivery capacity: In-vitro studies

Victoria Tamara Perchyonok
School of Dentistry and Oral Health, Griffith University, Gold Coast, Southport, QLD 4215, Australia
Shengmiao Zhang
1   School of Material Science and Engineering, East China University of Science and Technology, Shanghai 200237, China
Sias R. Grobler
2   Oral and Dental Research Institute, Faculty of Dentistry, University of the Western Cape, Private Bag X1, Tygerberg 7505, Cape Town, South Africa
Theunis G. Oberholzer
School of Dentistry and Oral Health, Griffith University, Gold Coast, Southport, QLD 4215, Australia
Ward Massey
School of Dentistry and Oral Health, Griffith University, Gold Coast, Southport, QLD 4215, Australia
› Author Affiliations


Background: Restorative materials in the new era aim to be “bio-active” and long-lasting. The purpose of this study was to design and to evaluate a novel chitosan hydrogels containing krill oil (antioxidant containing material), nystatin (antifungal), aspirin (pain relieve medication and free radical scavengers) and combinations thereof (chitosan-H-krill oil, chitosan-H-krill oil-nystatin and chitosan-H-aspirin, chitosan-H-aspirin-nystatin, chitosan-H-krill oil-aspirin and chitosan-H-krill oil-aspirin-nystatin) as functional additive prototypes for further development of “dual function restorative materials,” and secondly to determine their effect on the dentin bond strength of a composite. Materials and Methods: The above-mentioned hydrogels were prepared by dispersion the corresponding component in glycerol and acetic acid with the addition of chitosan gelling agent. The surface morphology (scanning electron microscope (SEM)), release behaviors (physiological pH and also in acidic conditions), stability of the therapeutic agent-antioxidant-chitosan and the effect of the hydrogels on the shear bond strength of dentin were also evaluated. Results: The release of nystatin and aspirin confer the added benefit of synergistic action of a functional therapeutic delivery when comparing the newly designed chitosan-based hydrogel restorative materials to the commercially available products alone. Neither the release of nystatin and aspirin nor the antioxidant stability was affected by storage over a 6 month period. The hydrogel formulations have a uniform distribution of drug content, homogenous texture and yellow color (SEM study). All chitosan dentin treated hydrogels gave significantly (P<0.05; non-parametric ANOVA test) higher shear bond values (P<0.05) than dentin treated or not treated with phosphoric acid. Conclusion: The added benefits of the chitosan treated hydrogels involved positive influence on the nystatin and aspirin release as well as increased dentin bond strength.

Publication History

Article published online:
01 November 2021

© 2014. European Journal of General Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (

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