Investigation of eluted monomers from resin-based root canal sealer by high-performance liquid chromatography analysis

ABSTRACT

Objective: The purpose of the current study was to determine the amount of urethane dimethacrylate (UDMA), bisphenol A-glycidyl methacrylate (Bis-GMA), poly (ethylene glycol) dimethacrylate (PEGDMA), bisphenol A ethoxylated dimethacrylate (Bis-EMA), and 2-hydroxyethyl methacrylate (HEMA) eluted from resin-based root canal sealer, epiphany, using high-performance liquid chromatography (HPLC). Materials and Methods: Epiphany was placed into the plastic molds and light-cured with a light emitting diode. After the curing process, each specimen in the first group (n = 12) was immersed in Eppendorf tubes containing a phosphate-buffered saline solution (PBS) and incubated for 45 s. In the second group, each specimen (n = 12) was immersed in Eppendorf tubes containing PBS and incubated for 24 h. Of the specimen extracts, 100 μL were subjected to HPLC. Analysis of data was accomplished with one-way analysis of variance (P < 0.05). Results: All of the samples eluted HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA. A significant difference was determined between the time periods of HEMA, UDMA, PEGDMA, and Bis-EMA (P < 0.05). Conclusion: The results of the current study showed that Epiphany releases HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA in both time periods.

Key words:

High-performance liquid chromatography - monomer - resin - sealer

• REFERENCES

• 1 Jorge JH, Giampaolo ET, Machado AL, Vergani CE. Cytotoxicity of denture base acrylic resins: A literature review. J Prosthet Dent 2003; 90: 190-3
• 2 Hensten-Pettersen A, Wictorin L. The cytotoxic effect of denture base polymers. Acta Odontol Scand 1981; 39: 101-6
• 3 Nakamura M, Kawahara H. Long-term biocompatibility test of denture base resins in vitro . J Prosthet Dent 1984; 52: 694-9
• 4 Lefebvre CA, Schuster GS, Caughman GB, Caughman WF. Effects of denture base resins on oral epithelial cells. Int J Prosthodont 1991; 4: 371-6
• 5 Moszner N, Ulrich S. New developments of polymeric dental composites. Prog Polym Sci 2001; 26: 535-76
• 6 Yoshii E. Cytotoxic effects of acrylates and methacrylates: Relationships of monomer structures and cytotoxicity. J Biomed Mater Res 1997; 37: 517-24
• 7 Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res 1998; 41: 474-80
• 8 Reichl FX, Simon S, Esters M, Seiss M, Kehe K, Kleinsasser N. et al. Cytotoxicity of dental composite (co) monomers and the amalgam component Hg(2+) in human gingival fibroblasts. Arch Toxicol 2006; 80: 465-72
• 9 Munksgaard EC, Peutzfeldt A, Asmussen E. Elution of TEGDMA and BisGMA from a resin and a resin composite cured with halogen or plasma light. Eur J Oral Sci 2000; 108: 341-5
• 10 Noda M, Komatsu H, Sano H. HPLC analysis of dental resin composites components. J Biomed Mater Res 1999; 47: 374-8
• 11 Moon HJ, Lim BS, Lee YK, Kim CW. Determination of residual monomers in dental pit and fissure sealants using food/oral simulating fluids. Bull Korean Chem Soc 2000; 21: 1115-8
• 12 Bagis YH, Rueggeberg FA. The effect of post-cure heating on residual, unreacted monomer in a commercial resin composite. Dent Mater 2000; 16: 244-7
• 13 Vaubert VM, Moon PC, Love BJ. Extractable free monomers from self-cured dental sealants resulting from dispensing errors. J Biomed Mater Res 1999; 48: 5-8
• 14 Dolez P, Marek M, Love BJ. Photopolymerizable acrylic resin: Effect of curing time and temperature. J Appl Polym Sci 2001; 82: 546-54
• 15 Hamid A, Hume WR. A study of component release from resin pit and fissure sealants in vitro . Dent Mater 1997; 13: 98-102
• 16 Tarumi H, Imazato S, Ehara A, Kato S, Ebi N, Ebisu S. Post-irradiation polymerization of composites containing bis-GMA and TEGDMA. Dent Mater 1999; 15: 238-42
• 17 Resende LM, Rached-Junior FJ, Versiani MA, Souza-Gabriel AE, Miranda CE, Silva-Sousa YT. et al. A comparative study of physicochemical properties of AH plus, epiphany, and epiphany SE root canal sealers. Int Endod J 2009; 42: 785-93
• 18 Karapinar KazandagM, Sunay H, Tanalp J, Bayirli G. Fracture resistance of roots using different canal filling systems. Int Endod J 2009; 42: 705-10
• 19 Monticelli F, Sword J, Martin RL, Schuster GS, Weller RN, Ferrari M. et al. Sealing properties of two contemporary single-cone obturation systems. Int Endod J 2007; 40: 374-85
• 20 Fisher MA, Berzins DW, Bahcall JK. An in vitro comparison of bond strength of various obturation materials to root canal dentin using a push-out test design. J Endod 2007; 33: 856-8
• 21 Monticelli F, Sadek FT, Schuster GS, Volkmann KR, Looney SW, Ferrari M. et al. Efficacy of two contemporary single-cone filling techniques in preventing bacterial leakage. J Endod 2007; 33: 310-3
• 22 Sjogren U, Hagglund B, Sundqvist G, Wing K. Factors affecting the long-term results of endodontic treatment. J Endod 1990; 16: 498-504
• 23 Orstavik D, Kerekes K, Eriksen HM. Clinical performance of three endodontic sealers. Endod Dent Traumatol 1987; 3: 178-86
• 24 Waltimo TM, Boiesen J, Eriksen HM, Ørstavik D. Clinical performance of 3 endodontic sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001; 92: 89-92
• 25 Pommel L, Camps J. In vitro apical leakage of system B compared with other filling techniques. J Endod 2001; 27: 449-51
• 26 Imazato S, McCabe JF, Tarumi H, Ehara A, Ebisu S. Degree of conversion of composites measured by DTA and FTIR. Dent Mater 2001; 17: 178-83
• 27 Wataha JC, Hanks CT, Strawn SE, Fat JC. Cytotoxicity of components of resins and other dental restorative materials. J Oral Rehabil 1994; 21: 453-62
• 28 Ak AT, Alpoz AR, Bayraktar O, Ertugrul F. Monomer Release from Resin Based Dental Materials Cured With LED and Halogen Lights. Eur J Dent 2010; 4: 34-40
• 29 Sideridou ID, Achilias DS. Elution study of unreacted Bis-GMA, TEGDMA, UDMA, and Bis-EMA from light-cured dental resins and resin composites using HPLC. J Biomed Mater Res B Appl Biomater 2005; 74: 617-26
• 30 Tabatabaee MH, Mahdavi H, Zandi S, Kharrazi MJ. HPLC analysis of eluted monomers from two composite resins cured with LED and halogen curing lights. J Biomed Mater Res B Appl Biomater 2009; 88: 191-6
• 31 Tanaka K, Taira M, Shintani H, Wakasa K, Yamaki M. Residual monomers (TEGDMA and Bis-GMA) of a set visible-light-cured dental composite resin when immersed in water. J Oral Rehabil 1991; 18: 353-62
• 32 Kawahara T, Nomura Y, Tanaka N, Teshima W, Okazaki M, Shintani H. Leachability of plasticizer and residual monomer from commercial temporary restorative resins. J Dent 2004; 32: 277-83
• 33 Altintas SH, Usumez A. Evaluation of TEGDMA leaching from four resin cements by HPLC. Eur J Dent 2012; 6: 255-62