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CC BY-NC-ND 4.0 · Eur J Dent 2014; 08(03): 402-406
DOI: 10.4103/1305-7456.137656
Original Article
Dental Investigation Society

Effect of erbium, chromium: yttrium, scandium, gallium, garnet laser and casein phosphopeptide-amorphous calcium phosphate on surface micro-hardness of primary tooth enamel

Priya Subramaniam
1   Department of Pedodontics and Preventive Dentistry, The Oxford Dental College, Hospital and Research Center, Bengaluru, Karnataka, India
,
Annu Pandey
1   Department of Pedodontics and Preventive Dentistry, The Oxford Dental College, Hospital and Research Center, Bengaluru, Karnataka, India
› Author Affiliations
Further Information

Publication History

Publication Date:
25 September 2019 (online)

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ABSTRACT

Objective: The aim was to evaluate the effect of Er, Cr: YSGG laser and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on surface micro-hardness of primary tooth enamel. Materials and Methods: A total of 30 freshly extracted caries free primary anterior teeth were cleaned and stored in 1% thymol. Teeth were embedded in acrylic resin such that only their buccal surfaces were exposed and were divided into four groups. Group I: Five intact teeth (negative control). The remaining 25 teeth were immersed for 30 min in 1% citric acid for demineralization. Group II: Five demineralized teeth (positive control), Group III: CPP-ACP (GC tooth mousse-GC International, Itabashi-Ku, Tokyo, Japan) application and Group IV: Etching using Er, Cr: YSGG laser + CPP-ACP application. Groups III and IV were subjected to pH cycling for 5 days. Surface micro-hardness of all the teeth was measured using Brinell hardness tester (Fuel Instruments and Engineers Pvt. Ltd.). Data were analyzed using ANOVA. Results: Mean surface micro-hardness of Groups I and II were 177.43 kgf/mm2 and 164.86 kgf/mm2, respectively. Group IV showed a higher mean surface micro-hardness (230.68 kgf/mm2) compared with that of Group III (190.28 kgf/mm2). In comparison to all other groups, laser etching prior to CPP-ACP application increased surface micro-hardness significantly (P < 0.001). Conclusion: Laser irradiation of primary teeth followed by CPP-ACP application increased surface micro-hardness of enamel.

Key words:

Casein phosphopeptide-amorphous calcium phosphate - erbium - chromium:yttrium - scandium - gallium - garnet laser - primary tooth enamel - re-mineralization surface hardness
 

  • REFERENCES

  • 1 Korytnicki D, Mayer MP, Daronch M, Singer Jda M, Grande RH. Effects of Nd: YAG laser on enamel microhardness and dental plaque composition: An in situ study. Photomed Laser Surg 2006; 24: 59-63
    CrossrefPubMedGoogle Scholar
  • 2 Hossain M, Nakamura Y, Kimura Y, Yamada Y, Kawanaka T, Matsumoto K. Effect of pulsed Nd: YAG laser irradiation on acid demineralization of enamel and dentin. J Clin Laser Med Surg 2001; 19: 105-8
    CrossrefPubMedGoogle Scholar
  • 3 Tsai CL, Lin YT, Huang ST, Chang HW. In vitro acid resistance of CO2 and Nd-YAG laser-treated human tooth enamel. Caries Res 2002; 36: 423-9
    CrossrefPubMedGoogle Scholar
  • 4 Lata S, Varghese NO, Varughese JM. Remineralization potential of fluoride and amorphous calcium phosphate-casein phospho peptide on enamel lesions: An in vitro comparative evaluation. J Conserv Dent 2010; 13: 42-6
    CrossrefPubMedGoogle Scholar
  • 5 Niazy AM, Ehab AH. Synergistic caries inhibitory effect of a remanerializing agent and CO2 laser on human enamel and root dentin. Cairo Dent J 2009; 25: 415-24
    Google Scholar
  • 6 Hicks MJ, Flaitz CM, Westerman GH, Blankenau RJ, Powell GL, Berg JH. Enamel caries initiation and progression following low fluence (energy) argon laser and fluoride treatment. J Clin Pediatr Dent 1995; 20: 9-13
    PubMedGoogle Scholar
  • 7 Hicks MJ, Westerman GH, Flaitz CM, Blankenau RJ, Powell GL, Berg JH. Effects of argon laser irradiation and acidulated phosphate fluoride on root caries. Am J Dent 1995; 8: 10-4
    PubMedGoogle Scholar
  • 8 Banda NR, Vanaja Reddy G, Shashikiran ND. Evaluation of primary tooth enamel surface morphology and microhardness after Nd: YAG laser irradiation and APF gel treatment – An in vitro study. J Clin Pediatr Dent 2011; 35: 377-82
    CrossrefPubMedGoogle Scholar
  • 9 Azevedo DT, Faraoni-Romano JJ, Derceli Jdos R, Palma-Dibb RG. Effect of Nd: YAG laser combined with fluoride on the prevention of primary tooth enamel demineralization. Braz Dent J 2012; 23: 104-9
    CrossrefPubMedGoogle Scholar
  • 10 Featherstone JD, Nobre dos Santos M, Fried D. Effect of a new carbon dioxide laser and fluoride on occlusal caries progression in dental enamel. Lasers Dent 2002; 4610: 132-9
    Google Scholar
  • 11 Apel C, Birker L, Meister J, Weiss C, Gutknecht N. The caries-preventive potential of subablative Er: YAG and Er: YSGG laser radiation in an intraoral model: A pilot study. Photomed Laser Surg 2004; 22: 312-7
    CrossrefPubMedGoogle Scholar
  • 12 Reynolds EC, Cai F, Shen P, Walker GD. Retention in plaque and remineralization of enamel lesions by various forms of calcium in a mouthrinse or sugar-free chewing gum. J Dent Res 2003; 82: 206-11
    Google Scholar
  • 13 Lennon AM, Pfeffer M, Buchalla W, Becker K, Lennon S, Attin T. Effect of a casein/calcium phosphate-containing tooth cream and fluoride on enamel erosion in vitro . Caries Res 2006; 40: 154-7
    CrossrefPubMedGoogle Scholar
  • 14 Cecchini RC, Zezell DM, de Oliveira E, de Freitas PM, Eduardo Cde P. Effect of Er: YAG laser on enamel acid resistance: Morphological and atomic spectrometry analysis. Lasers Surg Med 2005; 37: 366-72
    CrossrefPubMedGoogle Scholar
  • 15 Esteves-Oliveira M, Pasaporti C, Heussen N, Eduardo CP, Lampert F, Apel C. Rehardening of acid-softened enamel and prevention of enamel softening through CO2 laser irradiation. J Dent 2011; 39: 414-21
    CrossrefPubMedGoogle Scholar
  • 16 Karlinsey RL, Mackey AC, Blanken DD, Schwandt CS. Remineralization of eroded enamel lesions by simulated saliva in vitro . Open Dent J 2012; 6: 170-6
    CrossrefPubMedGoogle Scholar
  • 17 Newbrun E. Cariology. 3rd ed.. Chicago, Illinois: Quintessence Publishing Co., Inc.; 1989
    Google Scholar
  • 18 Craig RG, Powes JM. Restorative Dental Materials. 11th ed.. St. Louis: Mosby; 2002
    Google Scholar
  • 19 Zezell DM, Ana PA, Albero FG, Cury JA, Bach-Mann L. Effect of infrared lasers on chemical and crystalline properties of enamel. Caries Res 2009b 43: 192
    Google Scholar
  • 20 Rose RK. Effects of an anticariogenic casein phosphopeptide on calcium diffusion in streptococcal model dental plaques. Arch Oral Biol 2000; 45: 569-75
    CrossrefPubMedGoogle Scholar
  • 21 Shen P, Cai F, Nowicki A, Vincent J, Reynolds EC. Remineralization of enamel subsurface lesions by sugar-free chewing gum containing casein phosphopeptide-amorphous calcium phosphate. J Dent Res 2001; 80: 2066-70
    Google Scholar
  • 22 Ana P, Bachmann L, Zezell MD. Lasers effects on enamel for caries prevention. Laser Phys 2006; 16: 865-75
    CrossrefGoogle Scholar
  • 23 Featherstone JD, Nelson DG. Laser effects on dental hard tissues. Adv Dent Res 1987; 1: 21-6
    CrossrefPubMedGoogle Scholar
  • 24 Bachmann L, Craievich AF, Zezell DM. Crystalline structure of dental enamel after Ho: YLF laser irradiation. Arch Oral Biol 2004; 49: 923-9
    CrossrefPubMedGoogle Scholar
  • 25 Westerman GH, Hicks MJ, Flaitz CM, Powell GL, Blankenau RJ. Surface morphology of sound enamel after argon laser irradiation: An in vitro scanning electron microscopic study. J Clin Pediatr Dent 1996; 21: 55-9
    PubMedGoogle Scholar