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CC BY-NC-ND 4.0 · Eur J Dent 2014; 08(01): 118-123
DOI: 10.4103/1305-7456.126264
Original Article
Dental Investigation Society

Surface and mineral changes of enamel with different remineralizing agents in conjunction with carbon-dioxide laser

Ajit George Mohan
1   Director, Dental and Medical Centre, Cochin, Kerala, India
,
A. V. Rajesh Ebenezar
2   Department of Conservative Dentistry and Endodontics, SRM Dental College, Chennai, Tamil Nadu, India
,
Mohamed Fayas Ghani
3   Private Practitioner and Consultant Endodontist, Tanjore, Tamil Nadu, India
,
Leena Martina
4   Private Practitioner and Consultant Endodontist, Chennai, Tamil Nadu, India
,
Ashwin Narayanan
2   Department of Conservative Dentistry and Endodontics, SRM Dental College, Chennai, Tamil Nadu, India
,
Bejoy Mony
5   Departments of Conservative Dentistry and Endodontics, Annoor Dental College, Kerala, India
› Author Affiliations
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Publication History

Publication Date:
24 September 2019 (online)

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ABSTRACT

Aim: The aim of this study was to evaluate the surface/mineral changes on enamel before and after the application of acidulated phosphate fluoride (APF) gel, fluoride enhanced hydroxyapatite gel and propolis in conjunction with carbon-dioxide (CO2) laser. Materials and Methods: Crowns of 40 human maxillary central incisors were collected and were divided into four groups of 10 each: Topical fluoride application only, topical fluoride application followed by CO2 laser irradiation, CO2 laser irradiation followed by topical fluoride application and CO2 laser irradiation before and after topical fluoride application. The 10 crowns in each group was again sectioned into four equal parts of mesio-incisal, disto-incisal, mesio-cervical and disto-cervical sections rendering 40 samples in each group. Each group was again subdivided into four subgroups: Subgroup C - untreated enamel surface (control). Subgroup A - APF gel application, subgroup R - fluoride enhanced hydroxyapatite gel application and subgroup P - propolis application. The surface morphology of the test samples were analyzed by scanning electron microscopy and mineral changes by energy dispersion X-ray spectrophotometer. Results: Total mineral content is maximum in Group 4A (CO2 laser irradiation before and after APF gel application) and calcium/phosphate ratio is highest in Group 4R (CO2 laser irradiation before and after Remin-Pro application). Group 2A (APF gel application followed by CO2 laser irradiation) has the maximum fluoride retention. Conclusion: Laser irradiation of enamel through a topically applied APF gel is effective in the prophylaxis and management of dental caries.

Key words:

Acidulated phosphate fluoride gel - carbon-dioxide laser - fluoridated hydroxyapatite gel - propolis - total mineral content
 

  • REFERENCES

  • 1 Stern RH, Vahl J, Sognnaes RF. Lased enamel: Ultrastructural observations of pulsed carbon dioxide laser effects. J Dent Res 1972; 51: 455-60
    Google Scholar
  • 2 Stern RH, Sognnaes RF, Goodman F. Laser effect on in vitro enamel permeability and solubility. J Am Dent Assoc 1966; 73: 838-43
    CrossrefPubMedGoogle Scholar
  • 3 Yamamoto H, Ooya K. Potential of yttrium-aluminum-garnet laser in caries prevention. J Oral Pathol 1974; 3: 7-15
    CrossrefPubMedGoogle Scholar
  • 4 Yamamoto H, Sato K. Prevention of dental caries by acousto-optically Q-switched Nd: YAG laser irradiation. J Dent Res 1980; 59: 137
    Google Scholar
  • 5 Nelson DG, Shariati M, Glena R, Shields CP, Featherstone JD. Effect of pulsed low energy infrared laser irradiation on artificial caries-like lesion formation. Caries Res 1986; 20: 289-99
    CrossrefPubMedGoogle Scholar
  • 6 Nelson DG, Wefel JS, Jongebloed WL, Featherstone JD. Morphology, histology and crystallography of human dental enamel treated with pulsed low-energy infrared laser radiation. Caries Res 1987; 21: 411-26
    CrossrefPubMedGoogle Scholar
  • 7 Tagomori S, Morioka T. Combined effects of laser and fluoride on acid resistance of human dental enamel. Caries Res 1989; 23: 225-31
    CrossrefPubMedGoogle Scholar
  • 8 Oho T, Morioka T. A possible mechanism of acquired acid resistance of human dental enamel by laser irradiation. Caries Res 1990; 24: 86-92
    CrossrefPubMedGoogle Scholar
  • 9 Fox JL, Yu D, Otsuka M, Higuchi WI, Wong J, Powell G. Combined effects of laser irradiation and chemical inhibitors on the dissolution of dental enamel. Caries Res 1992; 26: 333-9
    CrossrefPubMedGoogle Scholar
  • 10 Kato IT, Kohara EK, Sarkis JE, Wetter NU. Effects of 960-nm diode laser irradiation on calcium solubility of dental enamel: An in vitro study. Photomed Laser Surg 2006; 24: 689-93
    CrossrefPubMedGoogle Scholar
  • 11 Hicks MJ, Flaitz CM, Westerman GH, Berg JH, Blankenau RL, Powell GL. Caries-like lesion initiation and progression in sound enamel following argon laser irradiation: An in vitro study. ASDC J Dent Child 1993; 60: 201-6
    PubMedGoogle Scholar
  • 12 Westerman GH, Hicks MJ, Flaitz CM, Blankenau RJ, Powell GL, Berg JH. Argon laser irradiation in root surface caries: In vitro study examines laser's effects. J Am Dent Assoc 1994; 125: 401-7
    CrossrefPubMedGoogle Scholar
  • 13 Westerman GH, Hicks MJ, Flaitz CM, Powell GL, Blankenau RJ. Surface morphology of sound enamel after argon laser irradiation: An in vitro scanning electron microscopic study. J Clin Pediatr Dent 1996; 21: 55-9
    PubMedGoogle Scholar
  • 14 Esteves-Oliveira M, Pasaporti C, Heussen N, Eduardo CP, Lampert F, Apel C. Prevention of toothbrushing abrasion of acid-softened enamel by CO (2) laser irradiation. J Dent 2011; 39: 604-11
    CrossrefPubMedGoogle Scholar
  • 15 Giamalia I, Steinberg D, Grobler S, Gedalia I. The effect of propolis exposure on microhardness of human enamel in vitro . J Oral Rehabil 1999; 26: 941-3
    CrossrefPubMedGoogle Scholar
  • 16 Anić I, Vidović D, Luić M, Tudja M. Laser induced molar tooth pulp chamber temperature changes. Caries Res 1992; 26: 165-9
    CrossrefPubMedGoogle Scholar
  • 17 Tepper SA, Zehnder M, Pajarola GF, Schmidlin PR. Increased fluoride uptake and acid resistance by CO2 laser-irradiation through topically applied fluoride on human enamel in vitro . J Dent 2004; 32: 635-41
    CrossrefPubMedGoogle Scholar
  • 18 Wu CC, Roan RT, Chen JH. Sintering mechanism of the CaF2 on hydroxyapatite by a 10.6-l microm CO2 laser. Lasers Surg Med 2002; 31: 333-8
    CrossrefPubMedGoogle Scholar
  • 19 Márquez F, Quintana E, Roca I, Salgado J. Physical-mechanical effects of Nd: YAG laser on the surface of sound dental enamel. Biomaterials 1993; 14: 313-6
    CrossrefPubMedGoogle Scholar
  • 20 Lee BS, Lin CP, Hung YL, Lan WH. Structural changes of Er: YAG laser-irradiated human dentin. Photomed Laser Surg 2004; 22: 330-4
    CrossrefPubMedGoogle Scholar
  • 21 Pai N, McIntyre J, Tadic N, Laparidis C. Comparative uptake of fluoride ion into enamel from various topical fluorides in vitro . Aust Dent J 2007; 52: 41-6
    CrossrefPubMedGoogle Scholar
  • 22 Featherstone JD, Barrett-Vespone NA, Fried D, Kantorowitz Z, Seka W. CO2 laser inhibitor of artificial caries-like lesion progression in dental enamel. J Dent Res 1998; 77: 1397-403
    Google Scholar