CC BY-NC-ND 4.0 · Eur J Dent 2013; 07(S 01): S026-S032
DOI: 10.4103/1305-7456.119060
Original Article
Dental Investigation Society

Pulp response to dentine adhesives: A study on mature human pulps

Pantelis Kouros
1   Department of Operative Dentistry, Faculty of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece
Eugenia Koliniotou-Koumpia
1   Department of Operative Dentistry, Faculty of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece
Elisabeth Koulaouzidou
1   Department of Operative Dentistry, Faculty of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece
Maria Helvatjoglu-Antoniades
1   Department of Operative Dentistry, Faculty of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece
Dimitrios Tziafas
2   Department of Endodontology, Faculty of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece
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25. September 2019 (online)


Objective: To clinically investigate the antibacterial effects of a commercially available self-etch 12-methacryloyloxy- dodecylpyridinium bromide (MDPB)-containing adhesive system in comparison with its respective non-MDPB-containing adhesive and to evaluate the pulp responses when in use on human teeth. Materials and Methods: Sixty-two viable human teeth scheduled for extraction were used. Class V cavities were prepared on the buccal surfaces of the teeth and filled with the tested materials (Protect Bond/Clearfil AP-X, SE Bond/Clearfil AP-X and Dycal/Ketac Fill Plus) as a control group, according to manufacturer′s recommendations. Randomly divided to two groups teeth remained intra-orally for 4 and 8 weeks. After extraction, teeth were decalcified, sectioned and stained using the Mayer′s hematoxylin and eosin, and modified Brown-Brenn′s technique. Pulp responses were evaluated microscopically under a microscope and remaining dentine thickness measured under a stereomicroscope. Results: No statistically significant differences regarding pulp inflammation or bacterial infiltration were found either for the materials tested or for periods of post-operative evaluation. Conclusions: The results suggested that for a short period of evaluation there are no quantitative differences, regardless to restoring material used.


  • 1 Anderson MH, Loesche WJ, Charbeneau GT. Bacteriologic study of a basic fuchsin caries-disclosing dye. J Prosthet Dent 1985; 54: 51-5
  • 2 Kidd EA, Banerjee A. What is absence of caries?. In: Albbrektsson T, Bratthall D, Per-Olof J, Glantz PO. editors. Tissue Preservation in Caries Treatment. New Malden, UK: Quintessence Publ. Co. Ltd.; 2001: p. 69-79
  • 3 Bergenholtz G. Evidence for bacterial causation of adverse pulpal responses in resin-based dental restorations. Crit Rev Oral Biol Med 2000; 11: 467-80
  • 4 Bergenholtz G, Cox CF, Loesche WJ, Syed SA. Bacterial leakage around dental restorations: Its effect on the dental pulp. J Oral Pathol 1982; 11: 439-50
  • 5 Imazato S. Antibacterial properties of resin composites and dentin bonding systems. Dent Mater 2003; 19: 449-57
  • 6 Imazato S, Kuramoto A, Kaneko T, Ebisu S, Russell RR. Comparison of antibacterial activity of simplified adhesive systems. Am J Dent 2002; 15: 356-60
  • 7 Imazato S, Kaneko T, Takahashi Y, Noiri Y, Ebisu S. In vivo antibacterial effects of dentin primer incorporating MDPB. Oper Dent 2004; 29: 369-75
  • 8 Imazato S. Bio-active restorative materials with antibacterial effects: New dimension of innovation in restorative dentistry. Dent Mater J 2009; 28: 11-9
  • 9 Goldberg M. In vitro and in vivo studies on the toxicity of dental resin components: A review. Clin Oral Investig 2008; 12: 1-8
  • 10 Tziafas D, Koliniotou-Koumpia E, Tziafa C, Papadimitriou S. Effects of a new antibacterial adhesive on the repair capacity of the pulp-dentine complex in infected teeth. Int Endod J 2007; 40: 58-66
  • 11 Ohmori K, Maeda N, Kohno A. Evaluation of antibacterial activity of three dentin primers using an in vitro tooth model. Oper Dent 1999; 24: 279-85
  • 12 Imazato S, Ehara A, Torii M, Ebisu S. Antibacterial activity of dentine primer containing MDPB after curing. J Dent 1998; 26: 267-71
  • 13 Gondim JO, Duque C, Hebling J, Giro EM. Influence of human dentine on the antibacterial activity of self-etching adhesive systems against cariogenic bacteria. J Dent 2008; 36: 241-8
  • 14 Stanley HR, Lundy T. Dycal therapy for pulp exposures. Oral Surg 1973; 35: 389-401
  • 15 Lee SJ, Walton RE, Osborne JW. Pulp response to bases and cavity depths. Am J Dent 1992; 5: 64-8
  • 16 Shovelton DS. A study of deep carious dentine. Int Dent J 1968; 18: 392-405
  • 17 Bouillaguet S, Virgillito M, Wataha J, Ciucchi B, Holz J. The influence of dentine permeability on cytotoxicity of four dentine bonding systems, in vitro . J Oral Rehabil 1998; 25: 45-51
  • 18 Murray PE, About I, Lumley PJ, Franquin JC, Remusat M, Smith AJ. Cavity remaining dentin thickness and pulpal activity. Am J Dent 2002; 15: 41-6
  • 19 Koliniotou-Koumpia E, Dionysopoulos P, Koumpia E. In vivo evaluation of microleakage from composites with new dentine adhesives. J Oral Rehabil 2004; 31: 1014-22
  • 20 Koliniotou-Koumpia E, Papadimitriou S, Tziafas D. Pulpal responses after application of current adhesive systems to deep cavities. Clin Oral Investig 2007; 11: 313-20
  • 21 Koliniotou-Koumpia E, Tziafas D. Pulpal responses following direct pulp capping of healthy dog teeth with dentine adhesive systems. J Dent 2005; 33: 639-47