CC BY-NC-ND 4.0 · Eur J Dent 2013; 07(03): 289-295
DOI: 10.4103/1305-7456.115412
Original Article
Dental Investigation Society

Self-etch bonding agent beneath sealant: Bond strength for laser-irradiated enamel

Maria Cristina Borsatto
1   Department of Pediatric Clinics, Preventive and Community Dentistry, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-904, Brazil.
Jackelline de Lemes Giuntini
1   Department of Pediatric Clinics, Preventive and Community Dentistry, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-904, Brazil.
Marta Maria Martins Giamatei Contente
1   Department of Pediatric Clinics, Preventive and Community Dentistry, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-904, Brazil.
Jaciara Miranda Gomes-Silva
1   Department of Pediatric Clinics, Preventive and Community Dentistry, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-904, Brazil.
Carolina Paes Torres
1   Department of Pediatric Clinics, Preventive and Community Dentistry, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-904, Brazil.
Rodrigo Galo
2   Department of Dental Materials and Prostheses, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-904, Brazil.
› Author Affiliations
Further Information

Publication History

Publication Date:
15 October 2019 (online)


Objectives: This study evaluated the in vitro shear bond strength (SBS) of a resin-based pit-and-fissure sealant (Fluroshield [F], Dentsply/Caulk) associated with either an etch-and-rinse (Adper Single Bond 2 [SB], 3M/ESPE) or a two-step self-etch adhesive system (Adper SE Plus [SE], 3M/ESPE) on Er: YAG laser-irradiated enamel. Materials and Methods: Seventeen sound third molar crowns were embedded in acrylic resin, and the mesial-distal enamel surfaces were flattened. The enamel sites were irradiated with a 2.94-μm wavelength Er: YAG laser (120 mJ, 4 Hz, noncontact mode/17 mm, 20 s). The specimens were randomly assigned to three groups according to the bonding technique: I - 37% phosphoric acid etching + SB + F; II - SE + F and III - F applied to acid-etched enamel, without an intermediate layer of bonding agent. In all of the groups, a 3-mm diameter enamel-bonding site was demarcated and the sealant cylinders were bonded. After 24 hours in distilled water, the shear bond strength was tested at a crosshead speed of 0.5 mm/minute. The data were analyzed by one-way ANOVA and Tukey′s test. The debonded specimens were examined with a stereomicroscope to assess the failure modes. Results: The mean SBS values in MPa were I = 6.39 (±1.44); II = 9.50 (±2.79); and III = 5.26 (±1.82). No statistically significant differences were observed between groups I and III; SE/F presented a significantly higher SBS than that of the other groups (P = 0.001). With regard to the failure mode, groups I (65%) and II (75%) presented adhesive failures, while group III showed 50% adhesive failure. Cohesive failure did not occur. Conclusion: The application of the two-step self-etch bonding agent (Adper SE Plus) beneath the resin pit-and-fissure sealant placement resulted in a significantly higher bond strength for the Er:YAG laser-irradiated enamel.


  • 1 Shaw L. Modern thoughts on fissure sealants. Dent Update 2000; 27: 370-4
  • 2 Jensen OE, Handelman SL. Effect of an autopolymerizing sealant on viability of microflora in occlusal dental caries. Scand J Dent Res 1980; 88: 382-8
  • 3 Simonsen RJ. Pit and fissure sealant: Review of the literature. Pediatr Dent 2002; 24: 393-414
  • 4 Feigal RJ. The use of pit and fissure sealants. Pediatr Dent 2002; 24: 415-22
  • 5 Feigal RJ, Hitt J, Splieth C. Retaining sealant on salivary contaminated enamel. J Am Dent Assoc 1993; 124: 88-97
  • 6 Bottenberg P, Graber HG, Lampert F. Penetration of etching agents and its influence on sealer penetration into fissures in vitro. Dent Mater 1996; 12: 96-102
  • 7 Garcia-Godoy F, Gwinnett AJ. An SEM study of fissure surfaces conditioned with a scraping technique. Clin Prev Dent 1987; 9: 9-13
  • 8 Feigal RJ, Musherure P, Gillespie B, Levy-Polack M, Quelhas I, Hebling J. Improved sealant retention with bonding agents: A clinical study of two-bottle and single-bottle systems. J Dent Res 2000; 79: 1850-6
  • 9 Feigal RJ, Quelhas I. Clinical trial of a self-etching adhesive for sealant application: Success at 24 months with Prompt L Pop. Am J Dent 2003; 16: 249-51
  • 10 Torres CP, Balbo P, Gomes-Silva JM, Ramos RP, Palma-Dibb RG, Borsatto MC. Effect of individual or simultaneous curing on sealant bond strength. J Dent Child 2005; 72: 31-5
  • 11 Borsatto MC, Corona SA, Alves AG, Chimello DT, Catirse AB, Palma-Dibb RG. Influence of salivary contamination on marginal microleakage of pit and fissure sealants. Am J Dent 2004; 17: 365-7
  • 12 Beauchamp J, Caufield PW, Crall JJ, Donly KJ, Feigal R, Gooch B. et al. Evidence-based clinical recommendations for the use of pit-andfissure sealants: A report of the American Dental Association Council on Scientific Affairs. J Am Dent Assoc 2008; 139: 257-68
  • 13 Kramer N, García-Godoy F, Lohbauer U, Schneider K, Assmann I, Frankenberger R. Preparation for invasive pit and fissure sealing: Air-abrasion or bur?. Am J Dent 2008; 21: 383-7
  • 14 Lepri TP, Souza-Gabriel AE, Atoui JA, Palma-Dibb RG, Pecora JD, Corona SA. Shear bond strength of a sealant to contaminated-enamel surface: Influence of erbium: Yttrium-aluminum-garnet laser pretreatment. J Esthet Restor Dent 2008; 20: 386-94
  • 15 Baygin O, Korkmaz FM, Tuzuner T, Tanriver M. The effect of different enamel surface treatments on the microleakage of fissure sealants. Laser Med Sci 2012; 27: 153-60
  • 16 Shahabi S, Bagheri HG, Ramazani K. Tensile bond strength of sealants following Er:YAG laser etching compared to acid etching in permanent teeth. Laser Med Sci 2012; 27: 371-5
  • 17 Hibst R, Keller U. Experimental studies of the application of the Er:YAG laser on dental hard substances: I. Measurement of the ablation rate. Lasers Surg Med 1989; 9: 338-44
  • 18 Hibst R, Keller U. The mechanism of Er: YAG laser induced ablation of dental hard substances. Proc Soc Photo Opt Instrum Eng 1993; 1880: 156-62
  • 19 Li ZZ, Code JE, Van de Merwe WP. Er: YAG laser ablation of enamel and dentin of human teeth: Determination of ablation rates at various fluences and pulse repetition rates. Lasers Surg Med 1992; 12: 625-30
  • 20 Lupi-Pegurier L, Marie-France B, Genovese O, Rocca JP, Muller-Bolla M. Microleakage of resin-based sealants after Er:YAG laser conditioning. Lasers Med Sci 2007; 22: 183-8
  • 21 Borsatto MC, Corona SA, Araújo FP, Souza-Gabriel AE, Pecora JD, Palma-Dibb RG. Effect of Er:YAG laser on tensile bond strength of sealants in primary teeth. J Dent Child 2007; 74: 104-8
  • 22 Perdigão J, Lopes M. Effect of conditioner and restorative resin on enamel bond strengths. Am J Dent 2000; 13: 88-92
  • 23 Perdigão J, Carmo AR, Anauate-Neto C, Amore R, Lewgoy HR, Cordeiro HJ. et al. Clinical performance of a selfetching adhesive at 18 months. Am J Dent 2005; 18: 135-40
  • 24 Marquezan M, da Silveira BL, Burnett Jr LH, Rodrigues CR, Kramer PF. Microtensile bond strength of contemporary adhesives to primary enamel and dentin. Clin Pediatr Dent 2008; 32: 127-32
  • 25 Venker DJ, Kuthy RA, Qian F, Kanellis MJJ. Twelve-month sealant retention in a school-based program using self-etching primer/adhesive. Public Health Dent 2004; 64: 191-7
  • 26 Hannig M, Grafe A, Atalay S, Bott B. Microleakage and SEM evaluation of fissure sealants by use of self-etching priming agents. J Dent 2004; 32: 75-81
  • 27 Al-Sarheed MA. Evaluation of shear bond strength and SEM observation of all-in one self-etching primer used for bonding of fissure sealants. J Contemp Dent Pract 2006; 7: 9-16
  • 28 Gomes-Silva JM, Torres CP, Contente MM, Oliveira MA, Palma-Dibb RG, Borsatto MC. Bond strength of a pit-and-fissure sealant associated to etch-and-rinse and self-etching adhesive systems to saliva-contaminated enamel: Individual vs. simultaneous light curing. Braz Dent J 2008; 19: 341-7
  • 29 Esteves-Oliveira M, Zezell DM, Apel C, Turbino ML, Aranha AC, Eduardo CP. Bond strength of self-etching primer to bur cut, Er, Cr:YSGG, and Er:YAG lased dental surfaces. Photomed Laser Surg 2007; 25: 373-80
  • 30 Van Landuyt KL, Kanumilli P, De Munck J, Peumans M, Lambrechts P, Van Meerbeek B. Bond strength of a mild self-etch adhesive with and without prior acid-etching. J Dent 2006; 34: 77-85
  • 31 De Munck J, Van Meerbeek B, Yudhira R, Lambrechts P, Vanherle G. Micro-tensile bond strength of two adhesives to Erbium: YAG-lased vs. bur-cut enamel and dentin. Eur J Oral Sci 2002; 110: 322-9
  • 32 Chimello-Sousa DT, de Souza AE, Chinelatti MA, Pecora JD, Palma-Dibb RG, Milori Corona SA. Influence of Er:YAG laser irradiation distance on the bond strength of a restorative system to enamel. J Dent 2006; 34: 245-51
  • 33 Castellan CS, Luiz AC, Bezinelli LM, Lopes RM, Mendes FM, Eduardo CP. et al. In vitro evaluation of enamel demineralization after Er:YAG and Nd:YAG laser irradiation on primary teeth. Photomed Laser Surg 2007; 25: 85-90
  • 34 Hossain M, Nakamura Y, Yamada Y, Ito M, Matsumoto K. Caries-preventive effect of Er:YAG laser irradiation with or without water mist. J Clin Laser Med Surg 2000; 18: 61-5
  • 35 Corrêa-Afonso AM, Ciccone-Nogueira JC, Pecora JD, Palma-Dibb RG. In vitro assessment of laser efficiency for caries prevention in pits and fissures. Microsc Res Tech 2012; 75: 245-52
  • 36 Maung NL, Wohland T, Hsu CY. Enamel diffusion modulated by Er:YAG laser (Part 2) Organic matrix. J Dent 2007; 35: 794-9
  • 37 Rodrigues LK, Nobre Dos Santos M, Pereira D, Assaf AV, Pardi V. Carbon dioxide laser in dental caries prevention. J Dent 2007; 32: 531-40