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CC BY-NC-ND 4.0 · Journal of Digestive Endoscopy 2014; 05(04): 144-148
DOI: 10.4103/0976-5042.150661
Original Article
Society of Gastrointestinal Endoscopy of India

Imprint cytology: A diagnostic aid in interpretation of upper gastrointestinal endoscopic biopsies

Divya Vijayanarasimha
Department of Pathology, JSS Medical College, Mysore, Karnataka, India
,
Asha Mahadevappa
Department of Pathology, JSS Medical College, Mysore, Karnataka, India
,
G. V. Manjunath
Department of Pathology, JSS Medical College, Mysore, Karnataka, India
,
R. Sunila
Department of Pathology, JSS Medical College, Mysore, Karnataka, India
› Author Affiliations
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Publication History

Publication Date:
26 September 2019 (online)

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Abstract

Context: Although major advances have occurred in the cytological diagnosis of various organ pathologies, gastrointestinal (GI) cytology has not gained popularity and is only occasionally practiced in the form of brushings and washings. Biopsy touch imprint cytology (IC) is a rapid, simple and inexpensive method of diagnosis of upper GI lesions. Thus, a study to show a correlation of IC and standard histopathology will allow the procedure to be performed routinely as adjunct to histopathology. Aims: To correlate results of imprints made from upper GI biopsies with histopathology in case of suspected upper GI lesions and to compare detection of Helicobacter pylori by IC and histopathology in suspected H. pylori infection. Subjects and Methods: One hundred and ten cases of upper GI endoscopic biopsies were included in the study. Touch imprints were made from the biopsies, stained and studied. The same tissue was put in 10% formalin for histopathological processing. Results of IC were compared with histopathology. Statistical Analysis Used: Chi-square/Fisher exact test were used to find out significance of study parameters. Results: Sensitivity and specificity of IC for neoplastic lesions were 94.3%, 100% for esophageal lesions; 88.2%, 97.14% for gastric lesions and 100%, 100% for duodenal lesions respectively, which correlated with other studies. Imprint smears for H. pylori had sensitivity of 80%, which was higher than that of brush cytology. Conclusions: IC is a valuable diagnostic tool and can be routinely applied as an adjunct to histopathological examination in the diagnosis of GI lesions.

Key words

Endoscopic biopsy - imprint cytology - upper gastrointestinal cytology
 

  • References

  • 1 Conrad R, ShobhaCastelino-Prabhu, Cobb C, Raza A. Role of cytopathology in the diagnosis and management of gastrointestinal tract cancers. J Gastrointest Oncol 2012;3:285-98.
  • 2 Sharma P, Misra V, Singh PA, Misra SP, Gupta SC. A correlative study of histology and imprint cytology in the diagnosis of gastrointestinal tract malignancies. Indian J Pathol Microbiol 1997;40:139-46.
  • 3 Mysorekar VV, Dandekar CP, Satyaprakash BS, Sarkar A. Role of imprint cytology in the diagnosis of gastrointestinal tract malignancies. Indian J Pathol Microbiol 2003;46:37-43.
  • 4 Misra SP, Dwivedi M, Misra V, Gupta SC. Imprint cytology – A cheap, rapid and effective method for diagnosing Helicobacter pylori. Postgrad Med J 1993;69:291-5.
  • 5 Geramizadeh B, Shafiee A, Saberfirruzi M, Kumar PK, Shaheem A. Brush cytology of gastric malignancies. Acta Cytol 2002;46:693-6.
  • 6 Hughes JH, Leigh CJ, Raab SS, Hook SY, Cohen MB, Suhrland MJ. Cytologic criteria for the brush diagnosis of gastric adenocarcinoma. Cancer 1998;84:289-94.
  • 7 Young JA, Hughes HE. Three year trial of endoscopic cytology of the stomach and duodenum. Gut 1980;21:241-6.
  • 8 Rosai J. Rosai and Ackerman’s Surgical Pathology. 10th ed. Noida: Elsevier; 2011.
  • 9 Yantiss RK. Diagnostic challenges in the pathologic evaluation of Barrett esophagus. Arch Pathol Lab Med 2010;134:1589-600.
  • 10 Kaur G, Madhavan M, Basri AH, Sain AH, Hussain MS, Yatiban MK, et al. Rapid diagnosis of Helicobacter pylori infection in gastric imprint smear. Med J Malaysia 2002;57:67.
  • 11 Rotimi O, Cairns A, Gray S, Moayyedi P, Dixon MF. Histological identification of Helicobacter pylori: Comparison of staining methods. J Clin Pathol 2000;53:756-9.
  • 12 Xu XQ, Wang ZH, Liao JX, Chen XY, Liu WZ, Xiao SD, et al. Predictive value of neutrophil infiltration as a marker of Helicobacter pylori infection. World J Gastroenterol 2012;18:5101-5.
  • 13 Sentürk O, Cantürk Z, Erçin C, Cantürk NZ, Celebi A, Hulagu S, et al. Comparison of five detection methods for Helicobacter pylori. Acta Cytol 2000;44:1010-4.
  • 14 Chang KJ, Katz KD, Durbin TE, Erickson RA, Butler JA, Lin F, et al. Endoscopic ultrasound-guided fine-needle aspiration. Gastrointest Endosc 1994;40:694-9.
  • 15 Stromar IK, Jakic-Razumovic J, Knezevic-Obad A, Knezevic J. Comparison of cytological and histological analysis of gastric mucosa biopsy in diagnosing malignant diseases. Lijec Vjesn 2004;126:287-90.