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DOI: 10.4103/0974-2727.72157
Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960
Source of Support: Nil
ABSTRACT
Tuberculosis is caused by Mycobacterium tuberculosis (M.tb) as well as Non-tubercular mycobacterium (NTM) with similar clinical presentation. Infections due to NTM are reported to have increased in the past few years. Growth of M.tb is inhibited by p-Nitrobenzoic acid (PNB), whereas, NTM are resistant. One hundred and nine isolates from various clinical samples were identified up to species level by their growth rate, pigmentation, and a battery of biochemical tests, including niacin accumulation, nitrate reduction, and heat-stable catalase (68°C) reactions. Para-nitrobenzoic acid (PNB) inhibition test was performed to differentiate between M.tb and NTM. PNB was added to the Lowenstein-Jensen (LJ) medium and BACTECTM MIGIT (Mycobacteria Growth Indicator Tube)960 medium to a final concentration of 500 μg/ml. All the M.tb isolates, including Mycobacterium tuberculosis H37Rv (standard strain), were inhibited by PNB on both LJ and MGIT 960. Of the NTM isolates, all were resistant to PNB on MGIT 960 and on LJ PNB, except one isolate of Mycobacterium marinum that was resistant to MGIT 960 PNB, but was susceptible to LJ PNB. The reporting time for M.tb ranged from 4–11 days (median 5.9 days) by MGIT 960 and for NTM it was 2–10 days with an average of 4.5 days. This study was carried out to establish the accuracy and efficiency of MGIT 960 PNB and to differentiate between M.tb and NTM.
Keywords
MGIT960 - mycobacterium tuberculosis - non-tubercular mycobacterium (NTM) - p-Nitro-benzoic acidPublication History
Article published online:
29 January 2020
© 2010.
Thieme Medical and Scientific Publishers Private Ltd.
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REFERENCES
- 1 Katoch VM. Infections due to non-tuberculous mycobacteria (NTM). Indian J Med Res 2004;120:290-304.
- 2 Cosivi O, Grange JM, Daborn CJ, Raviglione MC, Fujikura T, Cousins D, et al. Zoonotic tuberculosis due to M. bovis in developing countries. Emerg Infect Dis 1998;4:59-70.
- 3 American Thoracic Society - Diagnosis and treatment of disease caused by nontuberculous mycobacteria: Official statement of the American Thoracic Society. Am J Respir Crit Care Med 1997;156:S1-25.
- 4 Horsburgh CR Jr. Mycobacterium avium complex infection in the acquired immunodeficiency syndrome. N Engl J Med 1991;324:1332-8.
- 5 Gopinath K, Singh S. Non-tuberculous mycobacteria in TB-endemic countries: Are we neglecting the danger? PLoS Negl Trop Dis 2010;4:e615.
- 6 Gross WM and Hawkins JE. Radiometric selective inhibition tests for differentiation of mycobacterium tuberculosis, mycobacterium bovis, and other mycobacteria. J Clin Microbiol 1985;21:565-8.
- 7 Tsukamura M. Differentiation of mycobacteria by susceptibility to hydroxylamine and 8-azaguanine. J Bacteriol 1965;90:556-7.
- 8 Tsukamura M, Tsukamura S. Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis by p-nitrobenzoic acid susceptibility. Tubercle 1964;45:64-5.
- 9 Eidus L, Diena BB, Greenberg L. The tuberculostatic activity of intermediate compounds of chloramphenicol. Antibiot Chemother (Basel) 1959;9:491-6.
- 10 Laszlo A, Eidus L. Test for differentiation of M.tuberculosis and M. bovis from other mycobacteria. Can J Microbiol 1978;24:754-6.
- 11 Tanzil HO, Chatim A, Kurniawan AS, Hasrul H. The inhibitory activity of nitroxoline on different mycobacteria and nocardia. Am Rev Respir Dis 1973;108:147-8.
- 12 Tsukamura M, Tsukamura S. Differentiation of mycobacteria by susceptibility to nitrite and propylene glycol. Am Rev Respir Dis 1968;98:505-6.
- 13 Kent PT, Kubica GP. Public health mycobacteriology. A Guide for the level III laboratory. Atlanta GA: US Health Service Centers for Disease Control; 1985.
- 14 Tuberculosis Research Centre. Manual of Laboratory Methods: Bacteriology. Madras, India: Tuberculosis Research Centre; 1987.
- 15 Collins T, Levett PN. Radiometric studies on the use of selective inhibitors in the identification of Mycobacterium spp. J Med Microbiol 1989;30:175-81.
- 16 Giampaglia CM, Martins MC, Inumaru VT, Butuem IV, Telles MA. Evaluation of a rapid differentiation test for the Mycobacterium tuberculosis complex by selective inhibition with p-nitrobenzoic acid and thiophene-2-carboxylic acid hydrazide. Int J Tuberc Lung Dis 2005;2:206-9.
- 17 Monteiro PH, Martins MC, Ueki SY, Giampaglia CM, Telles MA. Cord formation and colony morphology for the presumptive identification of Mycobacterium tuberculosis complex. Braz J Microbiol 2003;34:171-4.
- 18 Tu HZ, Chang SH, Huaug TS, Huaug WK, Liu YC, Lee SS. Microscopic morphology in smears prepared from MGIT broth medium for rapid presumptive identification of Mycobacterium tuberculosis complex, Mycobacterium avium complex and Mycobacterium kansasii. Ann Clin Lab Sci 2003;33:179-83.
- 19 Giampaglia CM, Martins MC, Chimara E, Oliveira RS, Vieira GB, Marsico AG, et al. Differentiation of Mycobacterium tuberculosis from other mycobacteria with rho-nitrobenzoic acid using MGIT 960. Int J Tuberc Lung Dis 2007;11:803-7.
- 20 Medeiros RF, Melo JB, Caldas PC, Santos LM, Barreto AM. Utilização de agentes inibidores (PNB e TCH) para identificacao de M. tuberculosis no sistema MB/Bact. Um estudo preliminar. Laes and Haes 2001;131:202-4.