Download PDF
CC BY-NC-ND 4.0 · J Lab Physicians 2010; 2(02): 074-077
DOI: 10.4103/0974-2727.72153
Original Article

Prevalence of Pseudomonas aeruginosa in Post-operative Wound Infection in a Referral Hospital in Haryana, India

K Prabhat Ranjan
Department of Microbiology, Maharaja Agrasen Medical College and Hospital, Agroha (Hisar), Haryana, India
,
Neelima Ranjan
Department of Microbiology, Maharaja Agrasen Medical College and Hospital, Agroha (Hisar), Haryana, India
,
Satish K Bansal
Department of Surgery, Maharaja Agrasen Medical College and Hospital, Agroha (Hisar), Haryana, India
,
D R Arora
Department of Microbiology, Maharaja Agrasen Medical College and Hospital, Agroha (Hisar), Haryana, India
› Author Affiliations Source of Support: Nil
› Further Information
Also available at
  PDF Download Permissions and Reprints

ABSTRACT

Background: The objective of our study was to determine the prevalence of Pseudomonas aeruginosa in the isolates of postoperative wound and its susceptibility pattern to commonly used antibiotics.

Materials and Methods: During a 2-year period, specimens were received as postoperative wound swabs in Microbiology Laboratory, Maharaja Agrasen Medical College, Agroha (Hisar), Haryana, India.

Result: Of the 300 bacterial isolates, 89 (29.6%) were P. aeruginosa, followed by Escherichia coli (61, 20.3%), Klebsiella spp. (50, 16.6%), Staphylococcus aureus (43, 14.3%), Proteus spp. (19, 6.3%), Acinetobacter spp. (9, 3.0%), and Citrobacter freundii (2, 0.6%). There was no growth in 27 (9.0%) specimens.

Conclusion: P. aeruginosa isolation was higher in male patients and most common in the age group of 21-40 years. The susceptibility pattern showed the organism to be most commonly susceptible to imipenem, followed by meropenem, cefoperazone/sulbactam, ticarcillin/clavulanate, and amikacin.Keywords: Pseudomonas aeruginosa, postoperative wound, prevalence, nosocomial, antibiotic



Publication History

Article published online:
29 January 2020

© 2010.

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • REFERENCES

  • 1 Kirkland KB, Briggs JP, Trivette SL, Wilkinson WE, Sexton DJ. The impact of surgical-site infections in the 1990s: Attributable mortality, excess length of hospitalization, and extra costs. Infect Control Hosp Epidemiol 1999;20:725-30.
  • 2 Calvin M. Cutaneous wound repair. Wounds 1998;10:12-32.
  • 3 Khan JA, Iqbal Z, Rahman SU, Farzana K, Khan A. Report: Prevalence and resistance pattern of Pseudomonas aeruginosa against various antibiotics. Pak J Pharm Sci 2008;21:311-5.
  • 4 Bodey GP, Bolivar R, Fainstein V, Jadeja L Infections caused by Pseudomonas aeruginosa. Rev Infect Dis 1983;5:279-313.
  • 5 Livermore DM. Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare? Clin Infect Dis 2002;34:634-40.
  • 6 Mousa H. Aerobic, anaerobic and fungal burn wound infections. J Hosp Infect 1997;37:317-23.
  • 7 Heinzelmann M, M Scott, T Lam. Factors predisposing to bacterial invasion and infection. Am J Surgery 2002;183:179-90.
  • 8 National Nosocomial Infections Surveillance (NNIS) System. NNIS report, data summary from January 1992 to June 2002, issued August 2002. Am J Infect Control 2002;30:458-75.
  • 9 Leigh DA, Emmanuel FX, Sedgwick J, Dean R. Post-operative urinary tract infection and wound infection in women undergoing caesarean section: A comparison of two study periods in 1985 and 1987. J Hosp Infect 1990;15:107-16.
  • 10 Russell RC, Williams NS, Bulstrode CJ. Bailey and Love's Short Practice of Surgery. 23rd ed. Oxford Press; 2000. p. 87-98. USA.
  • 11 Andenaes K, Lingaas E, Amland PF, Giercksky KE, Abyholm F. Preoperative bacterial colonization and its influence on post operative wound infection in plastic surgery. J Hosp Infect 1996;34:291-9.
  • 12 Trilla A. Epidemiology of nosocomial infections in adult intensive care units. Intensive Care Med 1994;20:1-4.
  • 13 Bertrand XM, Thouverez C, Patry P, Balvay, Talon D. Pseudomonas aeruginosa: antibiotic susceptibility and genotypic characterization of strains isolated in the intensive care unit. Clin Microbiol Infect 2002;7:706-8.
  • 14 Forbes BA, Sahm DF, Weissfeld AS. Pseudomonas, Burkholderia, and similar organisms. In: Forbes BA, Sahm DF, Weissfeld AS, editors. Bailey and Scott’s Diagnostic Microbiology. 11th ed. St. Louis: Mosby Inc; 2002. p. 448-61.
  • 15 Central Laboratory Standards Institute (CLSI). Performance standards for antimicrobial disc susceptibility tests, Approved standards 10th ed. CLSI document M02-A10, Vol 29, No.1.
  • 16 Anupurba S, Bhattacharjee A, Garg A, Sen MR. Antimicrobial susceptibility of Pseudomonas aeruginosa from wound infections. Indian J Dermatol 2006;51:286-8.
  • 17 Oguntibeju OO, Nwobu RAU. Occurrence of Pseudomonas aeruginosa in post-operative wound infection. Pak J Med Sci 2004;20:187-92.
  • 18 Masaadeh HA, Jaran AS. Incident of Pseudomonas aeruginosa in post-operative wound infection. Am J Infect Dis 2009;5:1-6.
  • 19 Oguntibeju OO, Nwobu RAU. Occurrence of Pseudomonas aeruginosa in post-operative wound infection. Pak J Med Sci 2004;20:187-92.
  • 20 Masaadeh HA, Jaran AS. Incident of Pseudomonas aeruginosa in post-operative wound infection. Am J Infect Dis 2009;5:1-6.
  • 21 Siguan SS, Ang BS, Pala IM, Baclig RM. Aerobic Surgical Infection: surveillance on microbiological etiology and antimicrobial sensitivity pattern of commonly used antibiotics. Phil J Microbiol Infect Dis 1990;19:27-33.
  • 22 Navaneeth BV, Sridaran D, Sahay D, Belwadi MR. A preliminary study on metallo-beta-lactamase producing Pseudomonas aeruginosa in hospitalized patients. Indian J Med Res 2002;116:264-7.
  • 23 Bonfiglio G, Carciotto V, Russo G, Stefani S, Schito GC, Debbia E, et al. Antibiotic resistance in Pseudomonas aeruginosa: an Italian survey. J Antimicrob Chemother 1998;41:307-10.
  • 24 Goossens H. Susceptibility of multi-drug-resistant Pseudomonas aeruginosa in intensive care units: results from the European MYSTIC study group. Clin Microbiol Infect 2003;9:980-3.
  • 25 Kohler T, Michea-Hamzehpour M, Epp SF, Pechere JC. Carbapenem activities against Pseudomonas aeruginosa: respective contributions of OprD and efflux systems. Antimicrob Agents Chemother 1999;43:424-7.
  • 26 Lewis CM, Zervos MJ. Clinical manifestations of Enterococcal infection. Eur Clin Microbial Infec Dis 1990;9:111-7.