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CC BY 4.0 · AIMS Genet 2019; 06(03): 046-054
DOI: 10.3934/genet.2019.3.46
Research Article

Molecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae

Tahereh Karamzadeh
1   Research Center for Health Sciences, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran
,
Hamzeh Alipour
1   Research Center for Health Sciences, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran
2   Department of Medical Entomology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran
,
Marziae Shahriari-Namadi
2   Department of Medical Entomology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran
,
Abbasali Raz
3   Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
,
Kourosh Azizi
1   Research Center for Health Sciences, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran
,
Masoumeh Bagheri
2   Department of Medical Entomology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran
,
Mohammad D. Moemenbellah-Fard
1   Research Center for Health Sciences, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran
2   Department of Medical Entomology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran
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Abstract

Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5. It lied at the interval of 26–131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata. It could be applied to research activities implementing a new essential component design in wound healing.

Keywords

molecular profile - Netrin-1 UNC-5 receptor - Lucilia sericata - blowfly larvae


Publication History

Received: 19 June 2019

Accepted: 02 August 2019

Article published online:
31 March 2021

© 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
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