MiR-608 rs4919510 C > G polymorphism increased the risk of bladder cancer in an Iranian population

Mohammad Hashemi; Fatemeh Bizhani; Hiva Danesh; Behzad Narouie; Mehdi Sotoudeh; Mohammad Hadi Radfar; Mehdi Honarkar Ramezani; Gholamreza Bahari; Mohsen Taheri; Saeid Ghavami

doi:10.3934/genet.2016.4.212

AIMS Genetics, Table of Contents

CC BY 4.0 · AIMS Genet 2016; 03(04): 212-218
DOI: 10.3934/genet.2016.4.212

Research article

MiR-608 rs4919510 C > G polymorphism increased the risk of bladder cancer in an Iranian population

Mohammad Hashemi

¹Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

²Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

,

Fatemeh Bizhani

²Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

,

Hiva Danesh

²Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

,

Behzad Narouie

¹Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

³Urology and Nephrology Research Center; Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

,

Mehdi Sotoudeh

³Urology and Nephrology Research Center; Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

,

Mohammad Hadi Radfar

³Urology and Nephrology Research Center; Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

,

Mehdi Honarkar Ramezani

³Urology and Nephrology Research Center; Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

,

Gholamreza Bahari

²Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

,

Mohsen Taheri

⁴Genetic of non-communicable disease research Center, Zahedan University of Medical Sciences, Zahedan, Iran

,

Saeid Ghavami

⁵Department of Human Anatomy and Cell Science, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

› Author Affiliations

Abstract

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Abstract

MicroRNAs (miRNAs) participate in diverse biological pathways and may act as oncogenes or tumor suppressors. The single nucleotide polymorphisms (SNPs) in miRNAs potentially can alter miRNA-binding sites on target genes as well as affecting miRNAs expression. The present study aimed to evaluate the impact of miR-608 rs4919510 C > G variant on bladder cancer risk. This case-control study conducted on 233 bladder cancer patients and 252 healthy subjects. Genotyping of miR-608 rs4919510 was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Our findings showed that CG as well as CG + GG genotypes significantly increased the risk of bladder cancer (OR = 1.94, 95% CI = 1.28–2.94, p = 0.002, and OR = 1.90, 95% CI = 1.26–2.86, p = 0.002, respectively) compared to CC genotype. The G allele significantly increased the risk of bladder cancer compared to C allele (OR = 1.69, 95% CI = 1.17–2.45, p = 0.005). Our findings proposed that miR-608 polymorphism might be associated with increased risk of bladder cancer in a sample of Iranian population. Further large-scale studies with different ethnicities are needed to verify our findings.

Keywords

miR-608 - bladder cancer - polymorphism

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