High expression of anti-apoptotic genes in grade I and II meningiomas

Daniela Pretti da Cunha Tirapelli; Sarah Bomfim Menezes; Indira Maynart Franco; Isis Lacrose Lustosa; Andressa Romualdo Rodrigues; Paulo Cézar Novais; Antçnio César Mendes Santiago; Fernanda Maris Peria; Luciano Neder Serafini; Alexandre Magno da Nóbrega Marinho; Carlos Gilberto Carlotti Jr; Benedicto Oscar Colli; Luís Fernando Tirapelli

doi:10.1590/0004-282X20170027

Arquivos de Neuro-Psiquiatria, Table of Contents

CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2017; 75(04): 209-215
DOI: 10.1590/0004-282X20170027

ARTICLE

High expression of anti-apoptotic genes in grade I and II meningiomas

Alta expressão de genes anti-apoptóticos em meningiomas graus I e II

Authors

Daniela Pretti da Cunha Tirapelli

¹Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Ribeirão Preto SP, Brasil;
Sarah Bomfim Menezes

¹Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Ribeirão Preto SP, Brasil;
Indira Maynart Franco

¹Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Ribeirão Preto SP, Brasil;
Isis Lacrose Lustosa

¹Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Ribeirão Preto SP, Brasil;
Andressa Romualdo Rodrigues

¹Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Ribeirão Preto SP, Brasil;
Paulo Cézar Novais

²Universidade de Marília, Departamento de Odontologia, Marília SP, Brasil;
Antçnio César Mendes Santiago

¹Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Ribeirão Preto SP, Brasil;
Fernanda Maris Peria

³Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Medicina Interna, Ribeirão Preto SP, Brasil;
Luciano Neder Serafini

⁴Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Patologia e Medicina Legal, Ribeirão Preto SP, Brasil;
Alexandre Magno da Nóbrega Marinho

⁵Universidade Federal de Campina Grande, Unidade Acadêmica de Ciências Médicas, Campina Grande PB, Brasil;
Carlos Gilberto Carlotti Jr

¹Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Ribeirão Preto SP, Brasil;
Benedicto Oscar Colli

¹Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Ribeirão Preto SP, Brasil;
Luís Fernando Tirapelli

¹Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Ribeirão Preto SP, Brasil;

Abstract

ABSTRACT

One of the different genetic mechanisms involved in the carcinogenesis of meningiomas is influenced by interactions between proteins that induce and inhibit apoptosis.

Objective

To evaluate the expression of c-FLIP, XIAP, Bcl-2, caspase 3, 8 and 9, cytochrome c, APAF 1 and Smac/DIABLO genes related to apoptosis pathways.

Methods

The gene expression was evaluated in 30 meningiomas (WHO grades I and II) and in 10 normal samples (from arachnoid tissue) through PCR-RT.

Results

The results showed higher expression of anti-apoptotic genes in meningiomas when compared to the control group, which had a low expression of pro-apoptotic genes.

Conclusion

There is a possible block in the activation of caspases through the intrinsic apoptosis pathway in meningiomas. c-FLIP modulates caspase 8 and, by inhibiting its activation due to the lack of connection with the receiver, there is a block to the FAS activation of apoptosis by its extrinsic pathway.

RESUMO

Um dos diferentes mecanismos genéticos envolvidos na carcinogênese de meningiomas é influenciado por interações entre proteínas que induzem e inibem a apoptose.

Objetivos

Avaliar a expressão de c-FLIP, XIAP, Bcl-2, caspase 3, 8 e 9, citocromo C, APAF 1 e Smac/DIABLO, genes relacionados com as vias da apoptose.

Métodos

A expressão gênica foi avaliada em trinta amostras de meningiomas (OMS grau I e II) e em dez amostras normais (de aracnóide) por PCR em tempo real.

Resultados

Os resultados mostraram maior expressão de genes antiapoptóticos em meningiomas quando comparados com controle, em contraste com a menor expressão de genes próapoptóticos.

Conclusão

Há um possível bloqueio na ativação de caspases através da via intrínseca da apoptose em meningiomas. O c-FLIP modula a caspase 8 e, desse modo, inibindo a sua ativação pela ausência de ligação com o receptor, há um bloqueio na ativação de FAS pela via extrínseca da apoptose.

meningioma - carcinogenesis - apoptosis - gene expression

meningioma - carcinogênese - apoptose - expressão gênica

Full Text

References

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