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J Brachial Plex Peripher Nerve Inj 2009; 04(01): e109-e114
DOI: 10.1186/1749-7221-4-22
Research article
Senoglu et al; licensee BioMed Central Ltd.

Intraperitoneal Alpha-Lipoic Acid to prevent neural damage after crush injury to the rat sciatic nerve[*]

Mehmet Senoglu
1   Department of Neurosurgery, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey
,
Vedat Nacitarhan
2   Department of Physical Medicine and Rehabilitation, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey
,
Ergul Belge Kurutas
3   Department of Biochemistry, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey
,
Nimet Senoglu
4   Department of Anaesthesiology and Reanimation, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey
,
Idris Altun
1   Department of Neurosurgery, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey
,
Yalcin Atli
3   Department of Biochemistry, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey
,
Davut Ozbag
5   Department of Anatomy, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey
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Publikationsverlauf

01. September 2009

25. November 2009

Publikationsdatum:
18. September 2014 (online)

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Abstract

Objective Crush injury to the sciatic nerve causes oxidative stress. Alfa Lipoic acid (a-LA) is a neuroprotective metabolic antioxidant. This study was designed to investigate the antioxidant effects of pretreatment with a-LA on the crush injury of rat sciatic nerve.

Methods Forty rats were randomized into four groups. Group I and Group II received saline (2 ml, intraperitoneally) and a-LA (100 mg/kg, 2 ml, intraperitoneally) in the groups III and IV at the 24 and 1 hour prior to the crush injury. In groups II, III and IV, the left sciatic nerve was exposed and compressed for 60 seconds with a jeweler’s forceps. In Group I (n = 10), the sciatic nerve was explored but not crushed. In all groups of rats, superoxide dismutase (SOD) and catalase (CAT) activities, as well as malondialdehyde (MDA) levels were measured in samples of sciatic nerve tissue.

Results Compared to Group I, Group II had significantly decreased tissue SOD and CAT activities and elevated MDA levels indicating crush injury (p < 0.05). In the a-LA treatment groups (groups III and IV), tissue CAT and SOD activities were significantly increased and MDA levels significantly decreased at the first hour (p < 0.05) and on the 3rd day (p < 0.05). There was no significant difference between a-LA treatment groups (p > 0.05).

Conclusion A-LA administered before crush injury of the sciatic nerve showed significant protective effects against crush injury by decreasing the oxidative stress. A-LA should be considered in the treatment of peripheral nerve injuries, but further studies are needed to explain the mechanism of its neuroprotective effects.

*This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


 

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