Thromb Haemost 2016; 116(01): 103-114
DOI: 10.1160/TH16-02-0130
Cellular Haemostasis and Platelets
Schattauer GmbH

CD142+/CD61+, CD146+ and CD45+ microparticles predict cardiovascular events in high risk patients following a Mediterranean diet supplemented with nuts

Gemma Chiva-Blanch
1   Cardiovascular Research Center (CSIC-ICCC), Barcelona, Spain; Biomedical Research Institute Sant Pau (IIB-Sant Pau), Barcelona, Spain
,
Javier Crespo
1   Cardiovascular Research Center (CSIC-ICCC), Barcelona, Spain; Biomedical Research Institute Sant Pau (IIB-Sant Pau), Barcelona, Spain
,
Rosa Suades
1   Cardiovascular Research Center (CSIC-ICCC), Barcelona, Spain; Biomedical Research Institute Sant Pau (IIB-Sant Pau), Barcelona, Spain
,
Gemma Arderiu
1   Cardiovascular Research Center (CSIC-ICCC), Barcelona, Spain; Biomedical Research Institute Sant Pau (IIB-Sant Pau), Barcelona, Spain
,
Teresa Padro
1   Cardiovascular Research Center (CSIC-ICCC), Barcelona, Spain; Biomedical Research Institute Sant Pau (IIB-Sant Pau), Barcelona, Spain
,
Gemma Vilahur
1   Cardiovascular Research Center (CSIC-ICCC), Barcelona, Spain; Biomedical Research Institute Sant Pau (IIB-Sant Pau), Barcelona, Spain
,
Judith Cubedo
1   Cardiovascular Research Center (CSIC-ICCC), Barcelona, Spain; Biomedical Research Institute Sant Pau (IIB-Sant Pau), Barcelona, Spain
,
Dolores Corella
2   CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
3   Department of Preventive Medicine and Public Health, University of Valencia, Valencia, Spain
,
Jordi Salas-Salvadó
2   CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
4   Human Nutrition Unit, Hospital Universitari Sant Joan, Institut d’Investigacions Sanitàries Pere Virgili, Universitat Rovira i Virgili, Reus, Spain
,
Fernando Arós
2   CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
5   Department of Cardiology, University Hospital Araba, Vitoria, Spain
,
Miguel-Angel Martínez-González
2   CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
6   Department of Epidemiology and Public Health, University of Navarra, Pamplona, Spain
,
Emilio Ros
2   CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
7   Lipid Clinic, Endocrinology and Nutrition Service, Institut d’Investigacions Biomèdiques August Pi Sunyer (IDIBAPS), Hospital Clinic, University of Barcelona, Barcelona, Spain
,
Montse Fitó
2   CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
8   Cardiovascular Risk and Nutrition Research Group (Regicor Study Group), Institut Hospital del Mar d’Investigacions Mèdiques (IMIM), Barcelona, Spain
,
Ramon Estruch
2   CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
9   Department of Internal Medicine, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain
,
Lina Badimon
1   Cardiovascular Research Center (CSIC-ICCC), Barcelona, Spain; Biomedical Research Institute Sant Pau (IIB-Sant Pau), Barcelona, Spain
› Author Affiliations

Financial support: GC-B is a Sara Borrell Postdoctoral Fellow (CD13/00023) from Instituto de Salud Carlos III. This work has been possible thanks to funding received from the Spanish Ministry of Economy and Competitiveness (Plan Estatal de I+D+I 2013–2016, SAF2013–42962-R, to LB and SAF2012–40208 to GV), from the Cardiovascular Research Network of Instituto de Salud Carlos III (RIC, RD12/0042/0027 to LB) and from CIBER CB06/03 Fisiopatología de la Obesidad y la Nutrición of Instituto de Salud Carlos III, (CIBERobn, RD06/0045 to RE). All grants were co-financed by European Union Funds, Fondo Europeo de Desarrollo Regional (FEDER) “Una manera de hacer Europa”.
Further Information

Publication History

Received: 17 February 2016

Accepted after major revision: 26 March 2016

Publication Date:
27 November 2017 (online)

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Summary

Circulating microparticles (cMPs) are small phospholipid-rich microvesicles shed by activated cells that play a pivotal role in cell signalling related to the pathogenesis of atherothrombosis. We aimed to investigate the prognostic value of cMPs released from different vascular cells for cardiovascular event (CVE) presentation in asymptomatic patients at high cardiovascular risk factors under nutritional and pharmacologic treatment. This is a nested case-control study of 50 patients from the five-year follow-up prospective PREDIMED trial enrolled in the nuts arm of the Mediterranean diet (Med Diet-nuts). We randomly selected 25 patients who had suffered a CVE during follow-up and pair-matched them for sex, age, and classical CV risk factors to 25 patients who remained asymptomatic (no-CVE). Total Annexin V-(AV)+ cMPs and cMPs from cells of the vascular compartment were quantified by flow cytometry at baseline and after one year follow-up. MedDiet-nuts and pharmacological treatment neither modified levels nor source of MP shedding in CVE patients. However, no-CVE patients showed 40–86 % decreased total AV+, PAC-1+/AV+, CD61+/AV+, CD142+/CD61+/AV+, CD62P+/AV+, CD146+/AV+, CD63+/AV+ and CD11a+/AV+ cMPs at one year follow-up (p ≤ 0.046, all). CD142+/CD61+/AV+, CD146+/AV+ and CD45+/AV+ cMPs were decreased in no-CVE patients compared to CVE patients. A ROCcurve clustered model for CD142+/CD61+/AV+, CD45+/AV+ and CD146+/AV+ cMPs predicted a future CVE [p<0.0001, AUC=0.805 (0.672 to 0.938)]. In patients at high CV risk profile treated with a controlled MedDiet supplemented with nuts and receiving up-to-date CV drug treatment, reduced cMPs derived from activated platelets, leukocytes and endothelial cells are predictive of protection against CVE within the next four years.