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Thromb Haemost 2016; 116(02): 328-336
DOI: 10.1160/TH16-01-0071
New Technologies, Diagnostic Tools and Drugs
Schattauer GmbH

Diagnostic potential of plasma microRNA signatures in patients with deep-vein thrombosis

Xiao Wang
1   Center for Primary Health Care Research, Lund University/Region Skåne, Sweden
,
Kristina Sundquist
1   Center for Primary Health Care Research, Lund University/Region Skåne, Sweden
,
Johan L. Elf
2   Vascular Centers, Lund University, Malmö, University Hospital Malmö, Sweden
,
Karin Strandberg
3   Department of Clinical Chemistry, Lund University, Malmö, University Hospital, Malmö, Sweden
,
Peter J. Svensson
4   Department of Coagulation Disorders, Lund University, Malmö, University Hospital, Malmö, Sweden
,
Anna Hedelius
1   Center for Primary Health Care Research, Lund University/Region Skåne, Sweden
,
Karolina Palmér
1   Center for Primary Health Care Research, Lund University/Region Skåne, Sweden
,
Ashfaque A. Memon
1   Center for Primary Health Care Research, Lund University/Region Skåne, Sweden
,
Jan Sundquist
1   Center for Primary Health Care Research, Lund University/Region Skåne, Sweden
,
Bengt Zöller
1   Center for Primary Health Care Research, Lund University/Region Skåne, Sweden
› Author Affiliations Financial support: This work was supported by grants awarded to Bengt Zöller by the Swedish Heart-Lung Foundation, ALF funding from Region Skåne awarded to Bengt Zöller, Jan Sundquist, and Kristina Sundquist, and grants awarded to Bengt Zöller, Kristina Sundquist and Jan Sundquist by the Swedish Research Council.
Further Information

Publication History

Received: 29 January 2016

Accepted after major revision: 28 April 2016

Publication Date:
09 March 2018 (online)

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Summary

For excluding deep-vein thrombosis (DVT), a negative D-dimer and low clinical probability are used to rule out DVT. Circulating microRNAs (miRNAs) are stably present in the plasma, serum and other body fluids. Their diagnostic function has been investigated in many diseases but not in DVT. The aims of present study were to assess the diagnostic ability of plasma miRNAs in DVT and to examine their correlation with known markers of hypercoagulability, such as D-dimer and APC-PCI complex. Plasma samples were obtained from 238 patients (aged 16–95 years) with suspected DVT included in a prospective multicentre management study (SCORE). We first performed miRNA screening of plasma samples from three plasma pools containing plasma from 12 patients with DVT and three plasma pools containing plasma from 12 patients without DVT using a microRNA Ready-to-use PCR Panel comprising 742 miRNA primer sets. Thirteen miRNAs that differentially expressed were further investigated by quantitative real-time (qRT)-PCR in the entire cohort. The plasma level of miR-424–5p (p=0.01) were significantly higher, whereas the levels of miR-136–5p (p=0.03) were significantly lower in DVT patients compared to patients without DVT. Receiver-operating characteristic curve analysis showed the area under the curve (AUC) values of 0.63 for miR-424–5p and 0.60 for miR-136–5p. The plasma level of miR-424–5p was associated with both D-dimer and APC-PCI complex levels (p<0.0001 and p=0.001, respectively). In conclusions, these findings indicate that certain miRNAs are associated with DVT and markers of hypercoagulability, though their diagnostic abilities are probably too low.

Supplementary Material to this article is available online at www.thrombosis-online.com.

Keywords

Deep venous thrombosis - microRNA - diagnosis - D-dimer - APC-PCI complex

 

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