Impact of aspirin dose on adenosine diphosphate-mediated platelet activities

Antonio Tello-Montoliu; Estela Thano; Fabiana Rollini; Ronakkumar Patel; Ryan E. Wilson; Ana Muñiz-Lozano; Francesco Franchi; Andrew Darlington; Bhaloo Desai; Luis A. Guzman; Theodore A. Bass; Dominick J. Angiolillo

doi:10.1160/TH13-05-0400

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2013; 110(04): 777-784
DOI: 10.1160/TH13-05-0400

Platelets and Blood Cells

Schattauer GmbH

Impact of aspirin dose on adenosine diphosphate-mediated platelet activities

Results of an in vitro pilot investigation

Antonio Tello-Montoliu

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Estela Thano

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Fabiana Rollini

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Ronakkumar Patel

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Ryan E. Wilson

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Ana Muñiz-Lozano

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Francesco Franchi

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Andrew Darlington

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Bhaloo Desai

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Luis A. Guzman

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Theodore A. Bass

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

,

Dominick J. Angiolillo

¹University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA

› Author Affiliations

Abstract

Summary

Different aspirin dosing regimens have been suggested to impact outcomes when used in combination with adenosine diphosphate (ADP) P2Y¹² receptor antagonists. Prior investigations have shown that not only aspirin, but also potent ADP P2Y¹² receptor blockade can inhibit thromboxane A2-mediated platelet activation. The impact of aspirin dosing on ADP mediated platelet activities is unknown and represents the aim of this in vitro pilot pharmacodynamic (PD) investigation. Twenty-six patients with stable coronary artery disease on aspirin 81 mg/day and P2Y¹² naïve were enrolled. PD assessments were performed at baseline, while patients were on 81 mg/day aspirin and after switching to 325 mg/day for 7 ± 2 days with and without escalating concentrations (vehicle, 1, 3, and 10 μM) of prasugrel’s active metabolite (P-AM). PD assays included flow cytometric assessment of VASP to define the platelet reactivity index (PRI) and the Multiplate Analyzer (MEA) using multiple agonists [ADP, ADP + prostaglandin (PGE1), arachidonic acid (AA), and collagen]. Escalating P-AM concentrations showed incremental platelet P2Y¹² inhibition measured by VASP-PRI (p<0.001). However, there were no differences according to aspirin dosing regimen at any P-AM concentration (vehicle: p=0.899; 1 ïM: p=0.888; 3 ïM: p=0.524; 10 ïM: p=0.548). Similar findings were observed in purinergic markers assessed by MEA (ADP and ADP+PGE1). P-AM addition significantly reduced AA and collagen induced platelet aggregation (p<0.001 for all measures), irrespective of aspirin dose. In conclusion, aspirin dosing does not appear to affect PD measures of ADP-mediated platelet reactivity irrespective of the degree of P2Y¹² receptor blockade. P2Y¹² receptor blockade modulates platelet reactivity mediated by alternative activators.

Keywords

Aspirin - platelet function - purinergic pathway

Full Text

References

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