Platelet lysates stimulate angiogenesis, neurogenesis and neuroprotection after stroke

Yael Hayon; Olga Dashevsky; Ela Shai; David Varon; Ronen R. Leker

doi:10.1160/TH12-11-0875

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2013; 110(02): 323-330
DOI: 10.1160/TH12-11-0875

Platelets and Blood Cells

Schattauer GmbH

Platelet lysates stimulate angiogenesis, neurogenesis and neuroprotection after stroke

Yael Hayon

¹Department of Neurology, Peritz and Chantal Scheinberg Cerebrovascular Research Laboratory, Hadassah Ein Kerem, Jerusalem, Israel

,

Olga Dashevsky

²Department of Hematology, Coagulation Unit Hadassah University Hospital, Hadassah Ein Kerem, Jerusalem, Israel

,

Ela Shai

²Department of Hematology, Coagulation Unit Hadassah University Hospital, Hadassah Ein Kerem, Jerusalem, Israel

,

David Varon

²Department of Hematology, Coagulation Unit Hadassah University Hospital, Hadassah Ein Kerem, Jerusalem, Israel

,

Ronen R. Leker

¹Department of Neurology, Peritz and Chantal Scheinberg Cerebrovascular Research Laboratory, Hadassah Ein Kerem, Jerusalem, Israel

› Institutsangaben

Abstract

Summary

Platelets contain chemo-attractants and mitogens that have a major role in tissue repair. Therefore we hypothesised that tissue regeneration secondary to activation of endogenous neural stem cells (eNSC) can be enhanced by delivering platelets to the ischaemic brain. To examine these potential therapeutic effects we injected platelet-poor plasma (PPP), fibroblast growth factor (FGF2) and platelet lysate (PLT) to the lateral ventricles after permanent middle cerebral artery occlusion (PMCAO) in rats. The animals were tested with the neurological severity score, and infarct volumes were measured at 90 days post–PMCAO. Immunohistochemistry was used to determine the fate of newborn cells and to count blood vessels in the ischaemic brain. Platelets significantly increased eNSC proliferation and angiogenesis in the subventricular zone (SVZ) and in the peri-lesion cortex. Functional outcome was significantly improved and injury size was significantly reduced in rats treated with PLT suggesting additional neuroprotective effects. In conclusion, local delivery of PLT to the lateral ventricles induces angiogenesis, neurogenesis and neuroprotection and reduces behavioural deficits after brain ischaemia.

Keywords

Angiogenesis - neurogenesis - neuroprotection - platelets - cerebral ischaemia

Volltext

Referenzen

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