Determinants of platelet conjugate formation with polymorphonuclear leukocytes or monocytes in whole blood

Benedetta Izzi; Agnieszka Pampuch; Simona Costanzo; Branislav Vohnout; Licia Iacoviello; Chiara Cerletti; Giovanni de Gaetano

doi:10.1160/TH07-06-0383

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2007; 98(06): 1276-1284
DOI: 10.1160/TH07-06-0383

Platelets and Blood Cells

Schattauer GmbH

Determinants of platelet conjugate formation with polymorphonuclear leukocytes or monocytes in whole blood

Authors

Benedetta Izzi^*
Agnieszka Pampuch^*^#
Simona Costanzo

¹Research Laboratories, “John Paul II” Center for High Technology Research and Education in Biomedical Sciences, Catholic University, Campobasso, Italy
Branislav Vohnout

¹Research Laboratories, “John Paul II” Center for High Technology Research and Education in Biomedical Sciences, Catholic University, Campobasso, Italy
Licia Iacoviello

¹Research Laboratories, “John Paul II” Center for High Technology Research and Education in Biomedical Sciences, Catholic University, Campobasso, Italy
Chiara Cerletti

¹Research Laboratories, “John Paul II” Center for High Technology Research and Education in Biomedical Sciences, Catholic University, Campobasso, Italy
Giovanni de Gaetano

¹Research Laboratories, “John Paul II” Center for High Technology Research and Education in Biomedical Sciences, Catholic University, Campobasso, Italy

Abstract

Summary

Following preliminary in-vitro experiments, platelet-leukocyte conjugates and their determinants were evaluated in citrated whole blood from 349 subjects (209 women, age 16–92 years) randomly recruited from the general population. Platelet activation by ADP/collagen but not leukocyte stimulation by fMLP or LTB4 resulted in formation of platelet conjugates with PMN or monocytes. In the population study, mixed cell conjugates, platelet P-selectin and leukocyte CD11b were measured by flow cytometry both at baseline and after in-vitro stimulation with ADP/collagen. The latter significantly increased platelet conjugates with either PMN or monocytes, platelet P-selectin and leukocyte CD11b expression. Platelet count significantly correlated with platelet-PMN, platelet-monocyte conjugates and P-selectin both at baseline and upon stimulation. In all conditions, both conjugate levels correlated with each other, when adjusted for gender, age and platelet count. Age correlated with platelet-PMN conjugate numbers in basal and stimulated conditions and with basal P-selectin. ADP/collagen stimulation resulted in higher P-selectin and conjugates values in women. Among risk factors, a significant correlation was found between conjugate and glucose levels. In conclusion, the presence and formation in whole blood from a large population of plateletleukocyte conjugates reflects primary platelet – but not leukocyte – activation and varies with gender, age, platelet count and blood glucose.

Keywords

Platelet-leukocyte conjugates - platelet P-selectin - cell activation markers - flow cytometry - general population

Full Text

References

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