Thromb Haemost 2007; 97(05): 795-802
DOI: 10.1160/TH06-08-0466
Blood Coagulation, Fibrinolysis and Cellular Haemostasis
Schattauer GmbH

Effect of staphylokinase-derived nonadecapeptide on the activation of plasminogen

Kiyotaka Okada
1  Department of Physiology
,
Shigeru Ueshima
1  Department of Physiology
2  Department of Food Science and Nutrition, Kinki University School of Agriculture, Nara, Japan
,
Hiroyuki Matsuno
3  Department of Clinical Pathological Biochemistry, Doshisha Women’s College of Liberal Arts, Kyoto, Japan
,
Naoyuki Kawao
1  Department of Physiology
,
Chikako Okamoto
1  Department of Physiology
,
Masaki Tanaka
4  Department of Surgery, Kinki University School of Medicine, Osakasayama, Japan
,
Osamu Matsuo
1  Department of Physiology
› Author Affiliations
Further Information

Publication History

Received 25 August 2006

Accepted after resubmission 02 March 2007

Publication Date:
24 November 2017 (online)

Summary

Staphylokinase (SAK) expresses plasminogen activator (PA) activity by forming a complex with plasmin. The interaction between the plasmin-SAK complex and plasminogen was investigated using synthesized peptides, which were constructed according to the amino acid sequence of the SAK molecule.A synthetic nonadecapeptide (SAK22–40) corresponding to Glu22-Leu40 by the SAK molecule enhanced the activation of Glu-plasminogen by the plasmin-SAK complex.Analysis of IAsys resonant mirror biosensor showed that SAK22–40 bound to Glu-plasminogen.This binding was completely inhibited by IgG against the B-chain in the plasminogen molecule. But, this binding was not inhibited by IgG against lysine-binding sites (LBS) of the A-chain in the plasminogen molecule. The substitution of Lys35 with Ala in SAK22–40 did not enhance the activation of Glu-plasminogen by the plasmin-SAK complex. When SAK22–40 was administrated in a mouse thrombosis model, earlier recanalization was observed than in mice with vehicle administration. Thus, a newly synthesized peptide, SAK22–40 enhanced Glu-plasminogen activation and induced effective thrombolysis.