Antithrombotic effects of saratin on human atherosclerotic plaques

Gemma Vilahur; Xavier Duran; Oriol Juan-Babot; Laura Casaní; Lina Badimon

doi:10.1160/TH03-11-0687

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2004; 92(01): 191-200
DOI: 10.1160/TH03-11-0687

New Technologies and Diagnostic Tools

Schattauer GmbH

Antithrombotic effects of saratin on human atherosclerotic plaques

Authors

Gemma Vilahur

¹Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain
Xavier Duran

¹Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain
Oriol Juan-Babot

¹Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain
Laura Casaní

¹Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain
Lina Badimon

¹Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain

Abstract

Summary

Platelets play a primary role in thrombus formation after plaque rupture. Platelets recognize the exposed collagen via Von Willebrand factor (VWF) and become activated. Saratin, an inhibitor of the VWF-dependent binding of platelets to collagen, may reduce the thrombotic risk associated to atherosclerosis. Our objective was to evaluate the antithrombotic effects of local treatment with saratin on human atherosclerotic lesions. Thrombus formation was assessed by the deposition of ¹¹¹Inplatelets on different human atherosclerotic lesions under three local shear conditions (800,1700 and 3400/s) with blood derived from catheterized pigs. Human atherosclerotic lesions were locally treated with saratin (30 µg/ml) at 37ºC for 5 min and placed in the chamber. Under stenotic shear conditions of 800/s, saratin significantly (p<0.05) reduced platelet deposition triggered by human denuded vessel wall (44%), fatty streaks (47%), severely damaged vessel (50%) and atherosclerotic plaque (57%). Thrombus characterization by immunohistochemistry showed also a reduction in fibrin deposition in treated vessels. These results suggest that the local site-specific treatment with saratin inhibits atherosclerotic plaque thrombogenicity at haemodynamic conditions typical of moderately stenotic coronary arteries.

Keywords

Von Willebrand Factor - collagen - saratin - platelets - thrombosis

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Referenzen

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