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Planta Med 2009; 75 - P-7
DOI: 10.1055/s-2009-1216445

A Rapid Microdistillation Method for the Texas and Turkish Salvia Species and Their Genetic Profiles

N Techen 1, N Tabanca 2, B Demirci 3, JL Turner 4, C Pounders 5, G Akaydin 6, F Demirci 3, Z Pan 2, IA Khan 1, 7, DE Wedge 2, KHC Baser 3
  • 1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS, 38677, USA
  • 2USDA-ARS-NPURU, The University of Mississippi, University, MS, 38677, USA
  • 3Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470, Eskisehir, Turkey
  • 4Dallas Arboretum and Botanical Garden, Dallas, TX, 75218, USA
  • 5USDA-ARS, Southern Horticultural Laboratory, Poplarville, MS, 39470, USA
  • 6Department of Biology Education, Hacettepe University, 06532 Ankara, Turkey
  • 7Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA

The leaves of Salvia (Labiatae) species have a reputed use in traditional medicine. They are known as ‘ada cayi’ in Turkey and consumed as a hot drink. Sage leaves are used traditionally as a tonic, stimulant, carminative, antiseptic, for inflammations in the mouth and for infections in Turkey [1]. Salvia madrensis, Salvia longispicata x farinacea, Salvia greggii, Salvia roemeriana, Salvia farinaceae, Salvia leucantha, Salvia splendens, Salvia coccinea from Dallas Arboretum & Botanical Garden and Salvia candidissima, S. forskahlei, S. tchihatcheffii, S. wiedemanni, S. napifolia, S. cryptantha, S. fruticosa from Turkey were subjected to microdistillation technique and their chemical compositions were analyzed using both gas chromatography (GC-FID) and gas chromatography–mass spectrometry (GC-MS) techniques. The differences in chemical composition of 15 Salvia species will be presented in this study. Short Single Repeat (SSR) Microsatellite loci are highly informative genetic markers useful for population genetic studies, molecular breeding and parentage determination. Microsatellites, short nucleotide (1–6 bp) sequences, are the current DNA marker of choice because of their highly polymorphic distribution within the genome. In this study we also report the isolation and characterization of microsatellites from 15 Salvia species from Turkey and other countries. The utility of SSR loci as possible method in determining chemotype and authentication of plant species was evaluated and discussed. References: [1] Demirci B, Tabanca N, Baser KHC (2002) Flavour Fragr. J. 17: 54–58.