Planta Med 2008; 74(14): 1730-1734
DOI: 10.1055/s-2008-1081354
Original Paper
Natural Products Chemistry
© Georg Thieme Verlag KG Stuttgart · New York

Purification, NMR Study and Immunostimulating Property of a Fucogalactan from the Fruiting Bodies of Ganoderma lucidum

LiBin Ye1 , 2 , JingSong Zhang2 , Kan Zhou3 , Yan Yang2 , Shuai Zhou2 , Wei Jia2 , RuiXia Hao2 , YingJie Pan4
  • 1College of Life Sciences, Nanjing Agricultural University, Nanjing, P. R. China
  • 2Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China
  • 3Graduate School, Zhejiang Forestry University, Hangzhou, P. R. China
  • 4College of Food Sciences, Shanghai Fisheries University, Shanghai, P. R. China
Further Information

Publication History

Received: April 24, 2008 Revised: July 2, 2008

Accepted: July 9, 2008

Publication Date:
05 August 2008 (online)

Abstract

A water-soluble polysaccharide (LZ-D-1) was isolated from Ganoderma lucidum fruiting bodies by DEAE-Sepharose Fast Flow and Sephacryl S-300 high-resolution chromatography. The polysaccharide had a molecular weight of 2.8 × 104 Da and was mainly composed of L-Fuc, D-Glc and D-Gal in a molar ratio of ca.1 : 1 : 5. LZ-D-1 had a sugar content of ˜98.7 % as measured using the phenol-sulfuric acid method. On the basis of methylation analysis, FT-IR spectrum and NMR experiments, the structure of the repeating unit of the polysaccharide was established. LZ-D-1 had a backbone composed of 1,6-disubstituted α-D-galactopyranosyl, 1,2,6-trisubstituted α-D-galactopyranosyl and had a branch composed of fucose attached to O-2 of a 1,2,6-trisubstituted α-D-galactopyranosyl residue. At the same time, the polysaccharide contained some 1,6-disubstituted glucopyranosyl residues and 1,3-disubstituted glucopyranosyl residues. An immunostimulating experiment in vitro indicated that LZ-D-1 stimulated proliferation of mouse spleen lymphocytes.

Abbreviations

HPAEC:High-performance anion-exchangechromatography

PHA:Phytohaemagglutinin

PMAAs:Partially methylated alditol acetates

References

Prof. Dr. JingSong Zhang

Institute of Edible Fungi

Shanghai Academy ofAgricultural Sciences

Shanghai 201106

People’s Republic of China

Phone: +86-21-6220-1337

Fax: +86-21-6220-1337

Email: zhangjs8888@163.com

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